玉米胚乳细胞原生质体的分离与流式纯化

郭艳萍^*, 任成杰^*, 李志伟, 王文斌, 张仁和, 路海东, 刘建兴华, 薛吉全^*, 郭东伟^*

Isolation and Flow Purification of Endosperm Protoplast from Developing Seed of Maize

GUO Yan-Ping^**, REN Cheng-Jie^**, LI Zhi-Wei, WANG Wen-Bin, ZHANG Ren-He, LU Hai-Dong, LIU Jian-Chao, ZHANG Xing-Hua, XUE Ji-Quan^*, GUO Dong-Wei^*

图3 质膜稳定剂对原生质体分离的影响
Fig. 3 Effect of membrane stabilizer on protoplasts isolation Z1: 80% CaCl 2 +20% MES+0.5 mmol L -1 DTT; Z2: 100% CaCl 2 +25% MES +0.5 mmol L -1 DTT; Z3: 120% CaCl 2 +30% MES+0.5 mmol L -1 DTT; Z4: 150% CaCl 2 +35% MES +0.5 mmol L -1 DTT.