水稻几丁质酶基因的转录与表达特征

范伟^*, 李雪姣^*, 关明俐, 缪刘杨, 史佳楠, 窦世娟, 刘丽娟, 李莉云, 刘国振^*

Transcriptional and Translational Characterization of Rice Chitinase Genes

FAN Wei^**, LI Xue-Jiao^**, GUAN Ming-Li, MIAO Liu-Yang, SHI Jia-Nan, DOU Shi-Juan, LIU Li-Juan, LI Li-Yun, LIU Guo-Zhen^*

图4 水稻PR3家族几丁质酶蛋白质在叶片中的表达谱上部图片为相应CHIT特异抗体检测水稻叶片蛋白质的WB结果; 中部为用HSP抗体检测的WB结果示样品的等量加样; 下部为采集3次WB数据, 计算平均值和方差绘制的折线图, 纵坐标为相对信号强度, 横坐标与泳道对应。泳道1~9分别代表水稻的苗期1 cm、2 cm、5 cm、10 cm和15 cm地上部, 分蘖期叶片、孕穗期叶片、开花期叶片和成熟期叶片的总蛋白质样品。
Fig. 4 Expression patterns of rice PR3 family chitinase proteins in normal growth leaves Upper panels: WB detection of the expression of CHIT at different time points in rice leaves; Middle panels: WB detection of HSP expression in rice leaves to demonstrate equal loading; Lower panels: Lanes 1-9: Protein samples isolated from normal rice leaves at seedling stage 1 cm, 2 cm, 5 cm, 10 cm, and 15 cm, tillering stage, booting stage, flowering stage and mature stage, respectively.