水稻几丁质酶基因的转录与表达特征

范伟^*, 李雪姣^*, 关明俐, 缪刘杨, 史佳楠, 窦世娟, 刘丽娟, 李莉云, 刘国振^*

Transcriptional and Translational Characterization of Rice Chitinase Genes

FAN Wei^**, LI Xue-Jiao^**, GUAN Ming-Li, MIAO Liu-Yang, SHI Jia-Nan, DOU Shi-Juan, LIU Li-Juan, LI Li-Yun, LIU Guo-Zhen^*

图5 水稻PR3几丁质酶蛋白质在抗白叶枯病反应中的表达谱上部图片为相应CHIT特异抗体检测接种白叶枯病菌后不同时间点的水稻叶片蛋白质的WB结果; 中部为用HSP抗体检测的WB结果示样品的等量加样; 下部为采集3次WB数据, 计算平均值和方差绘制的折线图, 纵坐标为相对信号强度, 横坐标与泳道对应。泳道1~9分别为接种后0 h、2 h、8 h和1 d、2 d、3 d、5 d、7 d、10 d的蛋白质样品。
Fig. 5 Expression patterns of rice PR3 family chitinase proteins in the incompatible reactions between rice and Xoo Upper panels: WB detection of the expression of CHIT at different time points in the incompatible interaction between rice and Xoo ; Middle panels: WB detection of HSP expression in rice leaves to demonstrate equal loading; Lower panels: plot of average and standard deviation among three repeats of WB analysis. Lanes 1-9: protein samples isolated from rice leaves at 0 h, 2 h, 8 h, 1 d, 2 d, 3 d, 5 d, 7 d, and 10 d after inoculation with Xoo , respectively.