两个水稻品种镉积累相关基因表达及其分子调控机制

黄志熊^1,², 王飞娟², 蒋晗², 李志兰³, 丁艳菲², 江琼², 陶月良⁴, 朱诚^1,^2,^*

Comparison of Cadmium-Accumulation-Associated Genes Expression and Molecular Regulation Mechanism between Two Rice Cultivars (Oryza sativa L. subspeciesjaponica)

HUANG Zhi-Xiong^1,², WANG Fei-Juan², JIANG Han², LI Zhi-Lan³, DING Yan-Fei², JIANG Qiong², TAO Yue-Liang⁴, ZHU Cheng^1,^2,^*

图3 不同生长发育期的秀水110和秀水11叶片中 OsPCR1 基因、siRNA的表达水平和DNA甲基化水平提取5个不同生长发育期秀水110和秀水11倒数第2和第3叶基因组DNA和总RNA。利用McrBC-qRT-PCR检测 OsPCR1 外显子2甲基化水平, qRT-PCR检测 OsPCR1 基因表达水平和siRNA表达水平。相对 OsPCR1 外显子2甲基化水平的数据校准到等量未经酶切的基因组DNA。 OsPCR1 基因的表达水平校准到 OsUBQ5 。siRNA的表达水平校准到U6小核仁RNA。所有数据以各自营养生长期的样本为参照, 以平均值#cod#x000b1;标准误表示mean #cod#x000b1; SE n =3~8。 OsPCR1 : OsPCR1 相对表达水平; siRNA: siRNA相对表达水平; Unme: OsPCR1 外显子2相对未甲基化水平。标注及缩写同图1。
Fig. 3 OsPCR1 expression level, siRNA expression level, and OsPCR1 exon 2 methylation level in leaves of A Xiushui 110 and B Xiushui 11 at five developmental stages of rice Genomic DNA and total RNA were isolated from leaves previously described in Fig. 1 legend. OsPCR1 exon 2 methylation, OsPCR1 and siRNA expressions were determined by the methods of McrBC-qRT-PCR and qRT-PCR, respectively. Relative unmethylated OsPCR1 exon 2 level, OsPCR1 mRNA and siRNA expressions were normalized to equal amounts of undigested DNA samples, OsUBQ5 and U6 small nuclear RNA, respectively and relative to the vegetative samples. The data are presented as mean #cod#x000b1; SE n = 3 to n = 8. OsPCR1 : relative OsPCR1 expression level; siRNA: relative siRNA level; Unme: relative unmethylated OsPCR1 exon 2 level. Symbols and abbreviation are the same as those given in Fig. 1.