两个水稻品种镉积累相关基因表达及其分子调控机制

黄志熊^1,², 王飞娟², 蒋晗², 李志兰³, 丁艳菲², 江琼², 陶月良⁴, 朱诚^1,^2,^*

Comparison of Cadmium-Accumulation-Associated Genes Expression and Molecular Regulation Mechanism between Two Rice Cultivars (Oryza sativa L. subspeciesjaponica)

HUANG Zhi-Xiong^1,², WANG Fei-Juan², JIANG Han², LI Zhi-Lan³, DING Yan-Fei², JIANG Qiong², TAO Yue-Liang⁴, ZHU Cheng^1,^2,^*

图4 不同生长发育期的秀水110和秀水11叶片中 OsPCR1 基因外显子2甲基化水平提取5个不同生长发育期秀水110和秀水11倒数第2和第3叶基因组DNA。利用特异性的引物和McrBC-qRT-PCR技术检测 OsPCR1 基因外显子2甲基化水平。数据校准到等量未经酶切的DNA样本, 以各自秀水110的样本为参照定义为1, 以平均值#cod#x000b1;标准误表示mean #cod#x000b1; SE n = 3~4。A~E对应时期, 标注及缩写同图1。
Fig. 4 OsPCR1 exon 2 methylation in leaves of Xiushui 110 and Xiushui 11 at five developmental stages of rice Genomic DNA was isolated from leaves described in Fig. 1 legend. OsPCR1 exon 2 methylation was determined by the methods of McrBC-qRT-PCR with specific primers. The data were normalized to equal amount of undigested DNA samples and relative to Xiushui 110 samples, respectively. The data are presented as means #cod#x000b1; SE n = 3 and n = 4. The stages in individual figure, symbols and abbreviation are the same as those given in Fig. 1.