簇毛麦新型HMW-GS的序列分析及加工品质效应鉴定

杨华¹, 高翔^1,^2,^*, 陈其皎¹, 赵万春^1,^*, 董剑^1,², 李晓燕¹

Isolation, Characterization and Farinograph Analysis of Novel HMW-GSs fromDasypyrum villosum

YANG Hua¹, GAO Xiang^1,^2,^*, CHEN Qi-Jiao¹, ZHAO Wan-Chun^1,^*, DONG Jian^1,², LI Xiao-Yan¹

图4 KF887414、KF887415、KF887416和KF887417基因的诱导表达产物和种子HMW-GS的SDS-PAGE分析 M: 蛋白质分子质量标准Marker II。A图中, 1、3、5和7泳道为未诱导重组质粒表达产物; 2、4、6和8泳道为经IPTG诱导后的重组质粒的表达产物; 箭头示基因的表达产物。B图中, 泳道1为未诱导重组质粒表达产物; 2~5泳道为经IPTG诱导后的重组质粒的表达产物, 依次是 KF887414、KF887415、KF887416和KF887417基因; 泳道6为种子提取的HMW-GS; 箭头示目的蛋白。
Fig. 4 Analyses of expressed proteins of genes from KF887414 to KF887417 and comparison with seed HMW-GS using SDS-PAGE M: Protein ladder marker II. In panel A, lanes 1, 3, 5, and 7 show proteins of recombinant plasmid before induction and lanes 2, 4, 6, and 8 show proteins of recombinant plasmid after adding IPTG; the arrows show expression products. In panel B, lane 1 shows the protein of recombinant plasmid before induction; lanes 2, 3, 4, and 5 show proteins of recombinant plasmid KF887414, KF887415, KF887416, and KF887417 after adding IPTG, respectively; lane 6 shows HMW-GS extracted from Dasypyrum villosum seed; the arrows show target proteins.