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用核基质结合区(SAR)序列提高小麦最小表达框转基因表达的稳定性
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苏瑞波1,2, 陈明2,*, 徐兆师2, 李连城2, 马庆1,*, 马有志2 |
Improvement of Minimal Gene Cassette Expression Stability by Scaffold Attachment Region (SAR) Sequence in Wheat Transformation
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SU Rui-Bo 1,2, CHEN Ming 2,*, XU Zhao-Shi 2, LI Lian-Cheng 2, MA Qing 1,*, MA You-Zhi 2
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图3 pSGUS重组质粒的酶切 A: Kpn I和 Spe I双酶切pSGUS表达载体。M1: marker DL10000; 1: pSGUS质粒经 Kpn I和 Spe I酶切; 2: pSGUS质粒; M2: marker III。B: Sac I和 Bam H I双酶切pSGUS表达载体。M: marker III; 1~2: pSGUS质粒经 Sac I和 Bam H I酶切; 3: pSGUS质粒。 Fig. 3 Digested vector of recombinant plasmid pSGUS A: Vector pSGUS digested by Kpn I and Spe I. M1: marker DL10000; 1: pSGUS digested by Kpn I and Spe I; 2: pSGUS; M2: marker III. B: Vector pSGUS digested by Sac I and Bam H I. M: marker III; 1-2: pSGUS digested by Sac I and Bam H I; 3: pSGUS. |
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