用核基质结合区(SAR)序列提高小麦最小表达框转基因表达的稳定性

苏瑞波^1,², 陈明^2,^*, 徐兆师², 李连城², 马庆^1,^*, 马有志²

Improvement of Minimal Gene Cassette Expression Stability by Scaffold Attachment Region (SAR) Sequence in Wheat Transformation

SU Rui-Bo^1,², CHEN Ming^2,^*, XU Zhao-Shi², LI Lian-Cheng², MA Qing^1,^*, MA You-Zhi²

图5 T 0 代转 GUS 和pSGUS线性片段小麦PCR检测结果 A: 转 GUS 小麦PCR检测结果。1~2: 阳性植株; 3: 阴性对照; 4: 受体; 5: 质粒对照; M: DL2000。B: 转pSGUS小麦PCR检测结果; M: DL2000; 1~9阳性植株; 10: 阴性对照; 11: 受体; 12: 质粒对照。
Fig. 5 Results of PCR detection of transgenic wheat plants transformed with GUS and pSGUS fragments A: PCR detection of GUS transgenic plants. 1-2: positive plants; 3: negative plant; 4: wild type; 5: plasmid control; M: DL2000. B: PCR detection of pSGUS transgenic plants. M: DL2000; 1-9: positive plants; 10: negative plant; 11: wild type; 12: plasmid control.