用核基质结合区(SAR)序列提高小麦最小表达框转基因表达的稳定性

苏瑞波^1,², 陈明^2,^*, 徐兆师², 李连城², 马庆^1,^*, 马有志²

Improvement of Minimal Gene Cassette Expression Stability by Scaffold Attachment Region (SAR) Sequence in Wheat Transformation

SU Rui-Bo^1,², CHEN Ming^2,^*, XU Zhao-Shi², LI Lian-Cheng², MA Qing^1,^*, MA You-Zhi²

图7 转 GmDREB3 基因小麦分子检测结果 A: PCR检测。M: DL2000; 1~6: 阳性株系; 7: 水对照; 8: 受体对照; 9: 质粒对照。B: RT-PCR检测。M: DL2000; 1~5: 阳性株系; 6: 水对照; 7: 受体对照; 8: 质粒对照。C: 插入片段5′端完整性检测。M: marker III; 1~4: 阳性株系; 5: 水对照; 6: 受体对照; 7: 质粒对照。D: 插入片段3′端完整性检测。M: marker III; 1~4: 阳性株系; 5: 水对照; 6: 受体对照; 7: 质粒对照。
Fig. 7 Molecular detection results of GmDREB3 transgenic wheat A: PCR result. M: DL2000; 1-6: positive lines; 7: water control; 8: Jimai 22; 9: plasmid control. B: RT-PCR result. M: DL2000; 1-5: positive lines; 6: water control; 7: Jimai 22; 8: plasmid control. C: PCR result of 5′ terminal 5′ insertion fragment. M: Marker III; 1-4: positive lines; 5: water control; 6: Jimai 22; 7: plasmid control. D: PCR result of 3′ terminal of insertion fragment. M: marker III; 1-4: positive lines; 5: water control; 6: Jimai 22; 7: plasmid control.