芥菜锌指蛋白转录因子基因Bj26的克隆与鉴定
贾双伟1,*, 高英1,*, 赵开军1,*

Cloning and Characterization ofBrassica junceaZinc Finger Protein Transcription Factor GeneBj26
JIA Shuang-Wei1,**, GAO Ying1,**, ZHAO Kai-Jun1,*
图1 pGADT7-Bj26及空载体pGADT7与诱饵载体pBait1-AbAi及pBait1-m-AbAi间的酵母单杂交 A, B: pGADT7-Bj26和pGADT7质粒分别转入Y1H Gold [pBait1- AbAi]菌株后, 按1:1左和1:10右稀释后在SD-LeuAbA 0 A和SD-LeuAbA 550 B平板上生长; C, D: pGADT7-Bj26和pGADT7质粒分别转入Y1H Gold [pBait1-m-AbAi]菌株后, 按1:1左和1:10右稀释后在SD-LeuAbA 0 C和SD-LeuAbA 550 D平板上生长。
Fig. 1 Yeast one-hybrid assay between pGADT7-Bj26, pGADT7 and pBait1-AbAi or pBait1-m-AbAi in Y1HGold yeast strain The prey construct pGADT7-Bj26 and negative construct pGADT7 were transformed into Y1H Gold [pBait1-AbAi] A and B or Y1H Gold [pBait1-m-AbAi] C and D strains respectively. And positive transformants were determined by spotting serial dilutions 1:1 left, 1:10 right of yeast onto SD-LeuAbA 0 A and C and SD-LeuAbA 550 B and D.