芥菜锌指蛋白转录因子基因<italic>Bj26</italic>的克隆与鉴定

芥菜锌指蛋白转录因子基因Bj26的克隆与鉴定

贾双伟^1,^*, 高英^1,^*, 赵开军^1,^*

Cloning and Characterization ofBrassica junceaZinc Finger Protein Transcription Factor GeneBj26

JIA Shuang-Wei^1,^**, GAO Ying^1,^**, ZHAO Kai-Jun^1,^*

图5 Bj26与BjC-P真菌诱导核心序列特异性结合从而激活BjC-P的活性 A: BjC-P全长质粒P1, 真菌诱导关键区段质粒P16, P16对应真菌诱导核心元件GTAGTGACTCAT, -668 ~ -657中保守序列缺失质粒P53以及Bj26质粒的构建简图 [ 22 ] ; B: 各质粒在完全展开的约6周龄的本氏烟叶片单独及共注射后的GUS染色图; C: P16和P53分别与pBj26共注射后的GUS荧光定量分析图。不同质粒间的GUS活性通过LUC活性来校正误差, 每一个检测质粒注射本氏烟叶片时同时注射pBI121-LUCint质粒。柱形图显示P16和P53分别与PBj26共注射后的GUS活性与相应P16, P53单独注射后GUS活性之间的比值。结果为3次实验的平均值。
Fig. 5 Bj26 activates BjC-P by specific binding to the core fugal-responsive element A: schematic diagram of P1, P16, P53, and PBj26 [ 22 ] ; B: GUS histochemical staining in young and expanded symmetrical leaves of 6-week-old Nicotiana benthamiana leave after infiltration or co-infiltration of each plasmid; C: quantization of GUS activity in transiently transformed Nicotiana benthamiana leaf. GUS activity was normalized with the LUC activity. Nicotiana benthamiana leaf was co-infiltrated with each of the tested plasmids along with the construct pBI121-LUCint. Columns show the ratio of GUS activity of P16 and P53 co-infiltrated with PBj26 to that of P16 and P53 alone, respectively. Values are the mean #cod#x000b1; SD n =3.