土壤宏基因组中抗草甘膦新基因的克隆与转化水稻的研究

王云鹏^1,², 马景勇¹, 马瑞², 马建^1,^*, 刘文国^1,^2,^*

Cloning of New Herbicide Resistant Gene in Soil Metagenomics and Generation of Transgenic Rice Plants

WANG Yun-Peng^1,², MA Jing-Yong¹, MA Rui², MA Jian^1,^*, LIU Wen-Guo^1,^2,^*

图2 利用原核表达验证 soilEPSPS 基因的功能 A: 原核表达载体pEASY-syth、pSYTH-soilEPSPS、pSYTH-CP4的结构; B: 携带pEASY-syth、pSYTH-soilEPSPS、pSYTH-CP4的大肠杆菌在含有不同浓度草甘膦培养基中培养12 h后的OD值; C: 携带pEASY-syth、pSYTH-soilEPSPS、pSYTH-CP4的大肠杆菌在含200 mmol L -1 草甘膦平板上生长情况。
Fig. 2 Prokaryotic expression of soilEPSPS in E. coli A: structure of prokaryotic expression vector pEASY-syth, pSYTH-soilEPSPS and pSYTH-CP4; B: OD values of E. coli with pEASY-syth, pSYTH-soilEPSPS and pSYTH-CP4 cultured after 12 h in the media containing different concentrations of glyphosate; C: growth of E. coli carrying pEASY-syth, pSYTH-soilEPSPS, and pSYTH-CP4 cultured on the M9 medium containing 200 mmol L -1 glyphosate.