土壤宏基因组中抗草甘膦新基因的克隆与转化水稻的研究

王云鹏^1,², 马景勇¹, 马瑞², 马建^1,^*, 刘文国^1,^2,^*

Cloning of New Herbicide Resistant Gene in Soil Metagenomics and Generation of Transgenic Rice Plants

WANG Yun-Peng^1,², MA Jing-Yong¹, MA Rui², MA Jian^1,^*, LIU Wen-Guo^1,^2,^*

图3 植物表达载体的构建 A: 植物表达载体pAE35的T-DNA结构; B: pAE35的酶切鉴定。M: DNA分子量标准200 bp ladder; 1~2: Xho I & Nsi I酶切pAE35。
Fig. 3 Construction of plant expression vector A: structure of T-DNA region of plant expression vector pAE35; B: identification of pAE35 digested by endonucleases of Xho I & Nsi I. M: marker of 200 bp DNA ladder; lane 1-2: the fragments after disgestion.