390份小麦-黑麦种质材料主要农艺性状分析及优异材料的GISH与FISH鉴定

罗巧玲^1,⁴, 郑琪², 许云峰¹, 李立会³, 韩方普², 许红星¹, 李滨², 马朋涛¹, 安调过^1,^*

Main Agronomic Traits of 390 Wheat-Rye Derivatives and GISH/FISH Identification of Their Outstanding Materials

LUO Qiao-Ling^1,⁴, ZHENG Qi², XU Yun-Feng¹, LI Li-Hui³, HAN Fang-Pu², XU Hong-Xing¹, LI Bin², MA Peng-Tao¹, AN Diao-Guo^1,^*

图2 8份小麦-黑麦种质材料的GISH和mc-FISH鉴定 A~F依次为小麦-黑麦种质材料R1103、R1162、R2203、R2221、R1147和R1156。A1~F1是以黑麦基因组DNA为探针绿色, 中国春基因组DNA为封阻的GISH鉴定结果, 其中B1和D1中的十字星号示无端带的2条黑麦染色体, C1和D1中的箭头示1对黑麦的小染色体。 A2~F2是以重复序列p A s1 红色和p S c119.2 绿色为探针的mc-FISH鉴定结果, C2和D2中的问号示不能确定归属的染色体。
Fig. 2 Identification of GISH and mc-FISH of eight wheat-rye derivatives A-F are wheat-rye derivatives R1103, R1162, R2203, R2221, R1147, and R1156, respectively. A1-F1 are GISH results with rye genomic DNA as a probe green and Chinese Spring DNA as a blocker. The cross stars in B1 and D1 show the two rye chromosomes without GISH binding pattern, and the arrows in C1 and D1 show a pair of small rye chromosomes. A2-F2 are mc-FISH results on the same metaphase after GISH analysis by p A s1 red and p S c119.2 green simultaneously. The question marks in C2 and D2 show the undetermined chromosomes.