超长链多不饱和脂肪酸在棉花中的异源合成
刘江1,**, 马燕斌2,**, 孙全喜1,3, 吴霞2, 李雪滢1, 孙美红1, 李燕娥2, 李新征1,*, 亓宝秀1,*

Production of Very Long Chain Polyunsaturated Fatty Acids in Cotton
LIU Jiang1,**, MA Yan-Bin2,**, SUN Quan-Xi1,3, WU Xia2, LI Xue-Ying1, SUN Mei-Hong1, LI Yan-E2, LI Xin-Zheng1,*, QI Bao-Xiu1,*
图4 重组载体pAUX2-Δ9、pAUX2-Δ9Δ8、pAUX2-Δ9Δ8Δ5和pAUX2-Δ9Δ8Δ5Δ15的 Xba I酶切检测 所有的质粒都用 Xba I酶切, 并在0.8%的琼脂糖电泳上分离。a: pAUX3-E9D8D5载体的结构示意图; b: 中间载体的酶切检测, 红色箭头所指是载体主干3.9 kb; c: 各个独立表达盒的大小。
Fig. 4 Recombinant plasmids pAUX2-Δ9, pAUX2-Δ9Δ8, pAUX2-Δ9Δ8Δ5, and pAUX2-Δ9Δ8Δ5Δ15 digested by Xba I All the intermediate constructs were digested by Xba I and separated on a 0.8% agarose gel. a: diagram of pAUX2-Δ9Δ8Δ5Δ15, showing the arrangement and linkage of individual cassette; b: restriction digestion of all intermediate vectors. Red arrow indicates the position of the vector backbone 3.9 kb; c: sizes of individual inserts.