谷子逆境应答相关的钙依赖蛋白激酶基因<italic>SiCDPK1</italic>的克隆与表达

谷子逆境应答相关的钙依赖蛋白激酶基因SiCDPK1的克隆与表达

余琴鸯, 尹恒, 安利佳, 李文利^*

Cloning and Expression Analysis of a Calcium-Dependent Protein Kinase GeneSiCDPK1 inSetaria italica

YU Qin-Yang, YIN Heng, AN Li-Jia, LI Wen-Li^*

图3 SiCDPK1 基因序列及推导氨基酸序列分析阴影部分为蛋白激酶区, 加粗部分为钙离子结合区, 两者中间为自抑连接区, 其中虚线框内为自抑片段, 剩余部分为可变区。下画线显示的是EF hand结构域。序列开头框内为豆蔻酰化保守序列MGXXXSXX, 其中G为豆蔻酰化位点, C为棕榈酰化位点。
Fig. 3 Analysis of SiCDPK1 gene sequence and deduced amino acid sequence The shadow shows the protein kinase domain, and the bold shows the calmodulin-like domain. The junction domain is between the protein kinase domain and the calmodulin-like domain, in which the autoinhibitory domain is shown by dashed box. The rest is variable domain. EF hand is underlined. The myristoylation conserved domain is MGXXXSXX, which is shown by the box at the beginning of the text. G is myristoylation site and C is palmitoylation site.