玉米A亚族bZIP转录因子基因<italic>ZmbZIP81</italic>的克隆、表达与功能分析

玉米A亚族bZIP转录因子基因ZmbZIP81的克隆、表达与功能分析

王策^1,², 杨艳歌^2,³, 吕维涛^2,^*, 周春菊^1,^*, 孙冬梅³, 邓馨²

Cloning, Expression, and Functional Analysis of an A Subfamily bZIP Transcription Factor GeneZmbZIP81 in Maize

WANG Ce^1,², YANG Yan-Ge^2,³, L#cod#x000dc; Wei-Tao^2,^*, ZHOU Chun-Ju^1,^*, SUN Dong-Mei³, DENG Xin²

图5 ZmbZIP81 过表达载体示意图A和 35SS::ZmbZIP81 转基因株系的RT-PCR结果B p35SS : ZmbZIP81 基因启动子; pA35S : ZmbZIP81 基因终止子; Pat : 草甘膦抗性基因; Nos-p : 草甘膦抗性基因启动子; Nos-T : 草甘膦抗性基因终止子; attB1和attB2: LR反应重组位点; LB: 载体左边界; RB: 载体右边界。
Fig. 5 Structure of ZmbZIP81 -overexpressed vector A and analysis of 35SS::ZmbZIP81 transgenic lines B p35SS : promoter of ZmbZIP81 ; pA35S : terminator of ZmbZIP81 ; Pat : glyphosate resistance gene; Nos-p : promoter of glyphosate resistance gene; Nos-T : terminator of glyphosate resistance gene; attB1 and attB2: LR recombination sites; LB: left border; RB: right border.