水稻抗稻瘟病基因Pi-taPi-b多重PCR体系的构建与应用
姚姝, 刘燕清, 张亚东, 朱镇, 陈涛, 赵庆勇, 周丽慧赵春芳于新, 王才林*

Establishment and Application of Multiplex PCR System for Blast Resistance GenesPi-ta andPi-bin Rice
YAO Shu, LIU Yan-Qing, ZHANG Ya-Dong, ZHU Zhen, CHEN Tao, ZHAO Qing-Yong, ZHOU Li-Hui, ZHAO Chun-Fang, YU Xin, WANG Cai-Lin*
图1 Pi-ta 和 Pi-b 基因标记的多重PCR琼脂糖凝胶电泳图 M: DL2000; 1: 抗病对照嘉33; 2: 感病对照丽江新团黑谷; 3~12: 分别为南粳44、南粳45、武运粳7号、武运粳8号、武运粳21、武粳15、武香粳14、常农粳5号、盐稻9号和扬粳805。
Fig. 1 Multiplex PCR specific for genes Pi-ta and Pi-b M: DL2000; 1: resistant control Jia 33; 2: susceptible control Lijiangxintuanheigu; 3-12: tested rice varieties Nanjing 44, Nanjing 45, Nanjing 9108, Nanjing 46, Nanjing 5055, Wuyunjing 7, Wuyunjing 8, Wuyunjing 21, Wujing 15, and Wuxiangjing 14.