水稻抗稻瘟病基因Pi-taPi-b多重PCR体系的构建与应用
姚姝, 刘燕清, 张亚东, 朱镇, 陈涛, 赵庆勇, 周丽慧赵春芳于新, 王才林*

Establishment and Application of Multiplex PCR System for Blast Resistance GenesPi-ta andPi-bin Rice
YAO Shu, LIU Yan-Qing, ZHANG Ya-Dong, ZHU Zhen, CHEN Tao, ZHAO Qing-Yong, ZHOU Li-Hui, ZHAO Chun-Fang, YU Xin, WANG Cai-Lin*
图2 pi-ta 和 pi-b 基因标记的多重PCR琼脂糖凝胶电泳图 M: DL2000; 1: 抗病对照嘉33; 2: 感病对照丽江新团黑谷, 3~12: 分别为徐稻3号、徐稻4号、徐稻6号、镇稻88、淮稻5号、淮稻9号、连粳4号、连粳5号、连粳6号和连粳7号。
Fig. 2 Multiplex PCR specific for genes pi-ta and pi-b M: DL2000; 1: susceptible control variety Lijiangxintuanheigu; 2: resistant control variety Jia 33, 3-12: tested rice varieties Xudao 3, Xudao 4, Xudao 6, Zhendao 88, Huaidao 5, Huaidao 9, Lianjing 4, Lianjing 5, Lianjing 6, and Lianjing 7.