拟南芥下胚轴向光弯曲 P2SA2基因的克隆与功能鉴定
赵青平, 赵翔, 慕世超, 肖慧丽, 张骁*

Functional Analysis and Mapping of Gene P2SA2 Involved in Hypocotyl Phototropism of Arabidopsis thaliana
ZHAO Qing-Ping, ZHAO Xiang, MU Shi-Chao, XIAO Hui-Li, ZHANG Xiao*
图1 PHOT2介导拟南芥下胚轴向光弯曲下游调节子筛选 A: 从EMS诱变 phot1 突变体M 2 代群体中筛选强蓝光不敏感突变体; 红框: 疑似突变体 phot1p2sa1 ; 绿框: 疑似突变体 phot1p2sa2 ; 黄框: 疑似突变体 phot1p2sa3 ; 箭头: 照光方向、强度和处理时间; B: 100 µmol m -2 s -1 蓝光单侧处理12 h后不同基因型拟南芥下胚轴向光弯曲表型; C: 不同基因型拟南芥下胚轴向光弯曲度统计。图中每个数据分别来自3次独立重复试验, 大约19~24颗苗的平均值±标准误。其中1: WT; 2: phot1 ; 3: phot1p2sa1 ; 4: phot1p2sa2 ; 5: phot1p2sa3 。
Fig. 1 Isolation of regulator involved in PHOT2-mediated phototropism of hypocotyls in Arabidopsis A: HBL insensitive mutants from Arabidopsis M 2 mutagenesis population of phot1 mutants and selfing M 1 ; red box: phot1p2sa1 ; green box: phot1p2sa2 ; yellow box: phot1p2sa3 ; Arrows: direction of light irradiation, fluence rate and treated time; B: typical seedling phototropic curvature in indicated lines at 100 µmol m -2 s -1 blue light for 12 hours; C: hypocotyl curvatures were measured in indicated lines. 1: WT; 2: phot1 ; 3: phot1p2sa1 ; 4: phot1p2sa2 ; 5: phot1p2sa3 . Values are the means ± SD n =19-24.