甘蓝BoExo70A1与BoSEC3、BoExo84蛋白相互作用的
酵母双杂交检测
高启国1,*, 刘豫东1,*, 蒲全明1, 张林成1, 朱利泉2, 王小佳1

Interaction Analysis of Brassica oleracea L. BoExo70A1 with BoSEC3 and BoExo84 Proteins by Using Yeast Two-hybird System
GAO Qi-Guo1,**, LIU Yu-Dong1,**, PU Quan-Ming1, ZHANG Lin-Cheng1, ZHU Li-Quan2, WANG Xiao-Jia1
酵母重组质粒双酶切电泳图 M: Trans2K plus II DNA marker; 1: pGBKT7-BoExo70A1质粒 Nde I/ Sal I双酶切; 2: pGADT7-SEC3质粒 Sma I/ Bam H I双酶切; 3: pGADT7-BoSEC10质粒 Cla I/ Bam H I双酶切; 4: pGADT7-BoSEC15质粒 Nde I/ Eco R I双酶切; 5: pGADT7-BoExo84-N质粒 Eco R I/ Xho l I双酶切; 6: pGADT7-BoExo84-C质粒 Eco R I/ Xho l I双酶切。