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油菜乙酰乳酸合酶突变体S638N的酶学特性及其对ALS类除草剂的抗性
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胡茂龙, 浦惠明*, 龙卫华, 高建芹, 戚存扣, 张洁夫, 陈松 |
Enzymatic Characteristics of Acetolactate Synthase Mutant S638N in Brassica napus and Its Resistance to ALS Inhibitor Herbicides
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HU Mao-Long , PU Hui-Ming *, LONG Wei-Hua, GAO Jian-Qin, QI Cun-Kou, ZHANG Jie-Fu, CHEN Song
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pCold-II-Wt (左)和pCold-II-S638N (右)诱导表达的SDS-PAGE分析 M: 蛋白分子量标准; 1, 5: 诱导前样品; 2, 6: 诱导后超声沉淀; 3, 7: 诱导后超声上清液; 4, 8: 纯化的目的蛋白; 箭头表示目的蛋白。 |
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