油菜乙酰乳酸合酶突变体S638N的酶学特性及其对ALS类除草剂的抗性

胡茂龙, 浦惠明^*, 龙卫华, 高建芹, 戚存扣, 张洁夫, 陈松

Enzymatic Characteristics of Acetolactate Synthase Mutant S638N in Brassica napus and Its Resistance to ALS Inhibitor Herbicides

HU Mao-Long, PU Hui-Ming^*, LONG Wei-Hua, GAO Jian-Qin, QI Cun-Kou, ZHANG Jie-Fu, CHEN Song

ALS重组蛋白的 Western blot检测(抗体为His标签抗体) M: 蛋白分子量标准; 1: 诱导前pCold-II-Wt; 2: 诱导后pCold-II-Wt; 3: 诱导前pCold II对照质粒; 4: 诱导后pCold II对照质粒; 5: 诱导前pCold-II-S638N; 6: 诱导后pCold-II-S638N; 箭头表示目的蛋白。