基于棉花U6启动子的海岛棉CRISPR/Cas9基因组编辑体系的建立 |
李继洋, 雷建峰, 代培红, 姚瑞, 曲延英, 陈全家, 李月, 刘晓东 |
Establishment of CRISPR/Cas9 Genome Editing System Based on GbU6 Promoters in Cotton ( Gossypium barbadenseL.) |
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LI Ji-Yang, LEI Jian-Feng, DAI Pei-Hong, YAO Rui, QU Yan-Ying, CHEN Quan-Jia, LI Yue, LIU Xiao-Dong
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| 基因编辑载体的酶切鉴定 M1, M2: 2K plus DNA marker. A: M-1 to M-4 were Gb U6-5P:: GGB -sgRNA1-Cas9II Gb U6-5P:: GGB -sgRNA2-Cas9II, Gb U6-5P:: ERA1 -sgRNA1-Cas9II, and Gb U6-5P:: ERA1 -sgRNA2-Cas9II, respectively. B: 35-1 to 35-4 were Gb U6-5P:: GGB -sgRNA1-Cas9I, Gb U6-5P:: GGB -SgRNA2-Cas9I, Gb U6-5P:: ERA1 -sgRNA1-Cas9I, and Gb U6-5P:: ERA1 -sgRNA2-Cas9I. |
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