自花授粉诱导的甘蓝功能基因BoSPI的克隆与表达分析

蒲敏, 罗绍兰, 廉小平, 张贺翠, 白晓璟, 王玉奎, 左同鸿, 高启国, 任雪松, 朱利泉

Cloning and Expression Analysis of BoSPI Induced by Self-pollination in Brassica oleracea L. var. capitata

Min PU, Shao-Lan LUO, Xiao-Ping LIAN, He-Cui ZHANG, Xiao-Jing BAI, Yu-Kui WANG, Tong-Hong ZUO, Qi-Guo GAO, Xue-Song REN, Li-Quan ZHU

图3 BoSPI基因序列及其推导的氨基酸序列(A)、Ca2+结合核心结构分析(B)和BoSPI蛋白三维结构预测(C) A图中下画线指示4个EF-hand结构域, 红色字体表示12个氨基酸组成的Ca2+结合的核心区域; B图中下排数字表示对这12个氨基酸残基的编号, 数字0表示该核心区域上游第一个氨基酸残基。

Fig. 3 BoSPI cDNA sequence and deduced amino acid sequence (A), analysis of the core area of calcium ion binding (B) and predicted three-dimensional structure (C) for BoSPI protein The underlined sequence means four EF-hand domains and the read marker means the core area of calcium ion binding in Fig. A; the numbers indicate the numbers of the 12 amino acid residues of the core area of calcium ion binding and the digital 0 represents the first amino acid residue upstream of the core region in Fig. B.