BnA7HSP70分子伴侣结合蛋白超表达能够提高甘蓝型油菜耐旱性
万丽丽1,*(
), 王转茸2, 辛强2, 董发明2, 洪登峰2, 杨光圣2
), 王转茸2, 辛强2, 董发明2, 洪登峰2, 杨光圣2
Enhanced Accumulation of BnA7HSP70 Molecular Chaperone Binding Protein Improves Tolerance to Drought Stress in Transgenic Brassica napus
Li-Li WAN1,*(), Zhuan-Rong WANG2, Qiang XIN2, Fa-Ming DONG2, Deng-Feng HONG2, Guang-Sheng YANG2
), Zhuan-Rong WANG2, Qiang XIN2, Fa-Ming DONG2, Deng-Feng HONG2, Guang-Sheng YANG2
图1. BnA7HSP70-GFP融合蛋白在拟南芥原生质体中的定位
(A) BnA7HSP70和GFP融合后蛋白的亚细胞定位, 在488 nm下原生质体中细胞质显示绿色荧光; (B)在580 nm下同样原生质体中叶肉细胞显示红色荧光信号; (C)自然光照视野下细胞图; (D)自然光和荧光下的融合图片。
Fig. 1. Subcellular localization of BnA7HSP70 and GFP fusion protein in Arabidopsis protoplasts.
(A) Subcellular localization of full-length GFP fused with green fluorescent protein (GFP), the cytoplasm showed a green fluorescent signal at 488 nm. (B) The mesophyll cells showed a red fluorescent signal at 580 nm. (C) A bright-field image of protoplast cell; (D) A bright-field image and the merged image are shown at the bottom. Scale bars, 10 µm.
