BnA7HSP70分子伴侣结合蛋白超表达能够提高甘蓝型油菜耐旱性

万丽丽, 王转茸, 辛强, 董发明, 洪登峰, 杨光圣

Enhanced Accumulation of BnA7HSP70 Molecular Chaperone Binding Protein Improves Tolerance to Drought Stress in Transgenic Brassica napus

Li-Li WAN, Zhuan-Rong WANG, Qiang XIN, Fa-Ming DONG, Deng-Feng HONG, Guang-Sheng YANG

图5 超表达BnA7HSP70转基因油菜能够在断水10 d后具有较高持水能力 (A)转基因植株(OE2、OE3、OE7和OE8)与非转基因植株在干旱条件下的相对含水量; (B)对转基因和非转基因材料每个家系取样6~8片叶子, 测定叶绿素含量。Control: 正常生长条件下的野生型和超表达植株; Stress: 生长到4周的转基因和非转基因植株进行连续7 d干旱处理。

Fig. 5 Overexpression of BnA7HSP70 makes Brassica napus hold more water in soil pot without irrigation for 10 days (A) Relative water content (RWC) of wild type and BnA7HSP70 overexpressing seedling (OE2, OE3, OE7, and OE8) leaves under drought condition. (B) Chlorophyll a and b concentrations were calculated as described in the materials and methods 1.5.3 and combined to give the total chlorophyll concentration, each of which is a mean of the samples taken from 6-8 leaf disks in each pool. Control: the wild and OE plants grew in the irrigated condition; Stress: four-week old wild and OE plants were subjected to progressive drought for seven days.