BnA7HSP70分子伴侣结合蛋白超表达能够提高甘蓝型油菜耐旱性

万丽丽, 王转茸, 辛强, 董发明, 洪登峰, 杨光圣

Enhanced Accumulation of BnA7HSP70 Molecular Chaperone Binding Protein Improves Tolerance to Drought Stress in Transgenic Brassica napus

Li-Li WAN, Zhuan-Rong WANG, Qiang XIN, Fa-Ming DONG, Deng-Feng HONG, Guang-Sheng YANG

图7 BnA7HSP70超表达植株表现出对衣霉素诱导细胞死亡的耐受性 (A)转基因和非转基因植株种子和幼苗对2.5 µg mL-1或者5.0 µg mL-1衣霉素的敏感性实验。(B)~(C) Evans Blue染色方法测定转基因和非转基因植株在衣霉素处理后7 d、14 d的细胞死亡程度。Abs (600 nm)测定值反映死细胞含量, 每个实验重复3次。

Fig. 7 BnA7HSP70 overexpression increases resistance against tunicamycin (TUN)-induced cell death (A) Seeds and seedlings from overexpressed plants (OE) and untransformed wild-type (WT) plants were exposed to 2.5 µg mL-1 or 5.0 µg mL-1 tunicamycin. (B)-(C) Seedlings were monitored for the development of chlorosis and necrotic lesions, and cell viability were measured by the Evans blue dye method. Abs (600 nm) reflects the dead cell content. The values represent the average of three replicates (±SD).