BnA7HSP70分子伴侣结合蛋白超表达能够提高甘蓝型油菜耐旱性

万丽丽, 王转茸, 辛强, 董发明, 洪登峰, 杨光圣

Enhanced Accumulation of BnA7HSP70 Molecular Chaperone Binding Protein Improves Tolerance to Drought Stress in Transgenic Brassica napus

Li-Li WAN, Zhuan-Rong WANG, Qiang XIN, Fa-Ming DONG, Deng-Feng HONG, Guang-Sheng YANG

图8 衣霉素处理后转基因和非转基因植株内质网胁迫的标签基因的表达分析 取衣霉素处理0、24、48和72 h的非转基因和转基因植株的叶片, 分析内源BnA7HSP70、BnCNX1、BnNRP和BnBiP3基因的表达量。DMSO处理的植株为对照。BnCNX1、BnBiP3和BnNRP基因是内质网胁迫的标志基因。

Fig. 8 Gene expression analysis of senescence and cell death-associated genes in wild type and OE plants under tunicamycin treatment Total RNA were isolated from wild type and transgenic plant leaves at 0, 24, 48, and 72 h of treatment, and the endogenous BnA7HSP70, BnCNX1, BnBiP3, and BnNRP in wild type and OE transgenic plants treatment with tunicamycin and the control DMSO were monitored by qRT-PCR. The bars indicate the confidence interval (P < 0.05, n = 3). BnCNX1, BnBiP3, and BnNRP are ER stress markers.