基于高通量测序的大麦InDel标记开发及应用

徐婷婷, 汪巧玲, 邹淑琼, 狄佳春, 杨欣, 朱银, 赵涵, 颜伟

Development and application of InDel markers based on high throughput sequencing in barley

Ting-Ting XU, Qiao-Ling WANG, Shu-Qiong ZOU, Jia-Chun DI, Xin YANG, Yin ZHU, Han ZHAO, Wei YAN

表2 遗传距离统计结果

Table 2 Statistics of genetic distance

编号 No. 最小遗传距离 Minimum genetic distance 最大遗传距离 Maximum genetic distance 编号 No. 最小遗传距离 Minimum genetic distance 最大遗传距离 Maximum genetic distance 距离 Distance 对应种质编号 No. 距离 Distance 对应种质编号 No. 距离 Distance 对应种质编号 No. 距离 Distance 对应种质编号 No. X1 0 X2 0.70 X4 X145 0.18 X272 0.68 X15 X2 0 X1 0.70 X4 X146 0.08 X123 0.70 X107 X3 0.08 X25 0.73 X189 X147 0.13 X205 0.73 X254 X4 0.22 X29 0.70 X1 X148 0.15 X213 0.73 X262 X5 0.14 X279 0.64 X95 X149 0.08 X262 0.71 X148 X6 0.22 X31 0.73 X88 X150 0.08 X251 0.76 X189 X7 0.15 X107 0.75 X125 X151 0.10 X48 0.71 X107 X8 0.27 X9 0.74 X257 X152 0.17 X248 0.68 X114 X9 0.19 X7 0.71 X263 X153 0.13 X154 0.67 X33 X10 0.26 X205 0.71 X257 X154 0.13 X258 0.66 X107 X11 0.13 X137 0.70 X278 X155 0.15 X168 0.64 X88 X12 0.15 X68 0.70 X189 X156 0.16 X134 0.69 X95 X13 0.20 X15 0.68 X250 X157 0.14 X137 0.66 X113 X14 0.19 X53 0.73 X238 X158 0.19 X155 0.73 X88 X15 0.20 X13 0.77 X32 X159 0.13 X166 0.69 X107 X16 0.17 X162 0.68 X164 X160 0.21 X74 0.73 X139 X17 0.15 X148 0.69 X262 X161 0.16 X257 0.78 X94 X18 0.30 X242 0.68 X113 X162 0.13 X270 0.69 X189 X19 0.28 X111 0.62 X173 X163 0.21 X152 0.72 X282 X20 0.18 X226 0.68 X215 X164 0.22 X156 0.72 X176 X21 0.23 X29 0.69 X104 X165 0.19 X180 0.70 X101 X22 0.21 X171 0.70 X85 X166 0.06 X253 0.64 X4 X23 0.11 X198 0.72 X107 X167 0.06 X194 0.70 X95 X24 0.25 X20 0.68 X177 X168 0.08 X192 0.69 X15 X25 0.08 X3 0.72 X189 X169 0.05 X192 0.67 X97 X26 0.22 X13 0.67 X247 X170 0.13 X174 0.68 X107 X27 0.20 X162 0.58 X6 X171 0.21 X22 0.76 X88 X28 0.27 X114 0.67 X204 X172 0.13 X121 0.69 X88 X29 0.15 X107 0.73 X268 X173 0.32 X122 0.79 X284 X30 0.22 X80 0.66 X173 X174 0.13 X170 0.62 X106 X31 0.12 X212 0.69 X103 X175 0.13 X3 0.76 X173 X32 0.15 X44 0.77 X15 X176 0.18 X25 0.72 X107 X33 0.23 X207 0.67 X153 X177 0.30 X148 0.75 X221 X34 0.31 X28 0.68 X281 X178 0.23 X283 0.65 X221 X35 0.16 X3 0.69 X95 X179 0.21 X119 0.68 X221 X36 0.21 X82 0.68 X173 X180 0.13 X48 0.72 X107 X37 0.11 X46 0.77 X114 X181 0.18 X188 0.63 X94 X38 0.15 X279 0.68 X280 X182 0.14 X54 0.71 X106 X39 0.19 X230 0.64 X113 X183 0.25 X155 0.65 X80 X40 0.32 X66 0.69 X15 X184 0.15 X257 0.80 X94 X41 0.24 X163 0.65 X95 X185 0.21 X170 0.60 X29 X42 0.22 X57 0.71 X103 X186 0.17 X131 0.66 X107 X43 0.21 X148 0.69 X158 X187 0.18 X119 0.68 X89 X44 0.09 X67 0.70 X95 X188 0.18 X181 0.67 X65 X45 0.15 X166 0.65 X15 X189 0.24 X105 0.76 X150 X46 0.11 X37 0.76 X118 X190 0.26 X250 0.65 X9 X47 0.18 X48 0.73 X107 X191 0.08 X129 0.73 X231 X48 0.10 X151 0.72 X107 X192 0.05 X169 0.68 X97 X49 0.16 X198 0.69 X88 X193 0.16 X278 0.72 X117 X50 0.19 X123 0.72 X106 X194 0.06 X167 0.70 X95 X51 0.16 X37 0.67 X15 X195 0.13 X146 0.70 X15 X52 0.19 X84 0.75 X7 X196 0.23 X259 0.65 X106 X53 0.19 X14 0.73 X118 X197 0.14 X284 0.77 X173 X54 0 X198 0.72 X107 X198 0 X54 0.70 X107 X55 0.16 X201 0.73 X7 X199 0.22 X112 0.67 X247 X56 0.13 X235 0.69 X7 X200 0.22 X205 0.71 X247 X57 0.19 X37 0.72 X94 X201 0.06 X216 0.68 X107 X58 0.19 X54 0.66 X189 X202 0.14 X218 0.66 X88 X59 0.20 X268 0.66 X149 X203 0.09 X213 0.71 X173 X60 0.14 X223 0.68 X107 X204 0.17 X162 0.73 X276 X61 0.11 X91 0.65 X177 X205 0.13 X147 0.83 X257 X62 0.19 X234 0.75 X94 X206 0.19 X107 0.70 X125 X63 0.21 X112 0.61 X273 X207 0.06 X208 0.66 X52 X64 0.23 X5 0.66 X189 X208 0.06 X207 0.61 X173 X65 0.19 X233 0.72 X7 X209 0.21 X111 0.66 X77 X66 0.14 X46 0.71 X106 X210 0.23 X107 0.67 X125 X67 0.09 X44 0.72 X88 X211 0.21 X136 0.65 X135 X68 0.15 X72 0.65 X88 X212 0.12 X31 0.70 X95 X69 0.13 X72 0.60 X164 X213 0.09 X203 0.71 X173 X70 0.22 X69 0.64 X280 X214 0.18 X208 0.63 X204 X71 0.04 X73 0.69 X107 X215 0.27 X118 0.70 X182 X72 0.12 X198 0.63 X280 X216 0.06 X201 0.67 X107 X73 0.04 X71 0.69 X107 X217 0.29 X170 0.66 X114 X74 0.16 X198 0.71 X189 X218 0.03 X54 0.68 X107 X75 0.09 X198 0.66 X105 X219 0.19 X98 0.70 X247 X76 0.20 X87 0.74 X257 X220 0.23 X88 0.66 X263 X77 0.08 X235 0.71 X107 X221 0.18 X225 0.75 X177 X78 0.13 X225 0.69 X7 X222 0.14 X123 0.71 X15 X79 0.19 X243 0.69 X106 X223 0.13 X242 0.68 X107 X80 0.20 X38 0.69 X114 X224 0.14 X198 0.63 X107 X81 0.24 X246 0.76 X88 X225 0.11 X225 0.66 X278 X82 0.14 X84 0.70 X164 X226 0.06 X54 0.66 X107 X83 0.19 X69 0.66 X269 X227 0.25 X224 0.75 X255 X84 0.09 X73 0.71 X177 X228 0.26 X188 0.68 X177 X85 0.16 X225 0.70 X22 X229 0.17 X195 0.67 X114 X86 0.28 X154 0.63 X81 X230 0.14 X198 0.69 X189 X87 0.20 X76 0.65 X247 X231 0.16 X263 0.73 X191 X88 0.11 X89 0.76 X81 X232 0.15 X274 0.75 X114 X89 0.11 X88 0.77 X269 X233 0.13 X84 0.63 X189 X90 0.20 X94 0.71 X257 X234 0.10 X44 0.76 X94 X91 0.11 X61 0.64 X264 X235 0.08 X77 0.69 X107 X92 0.27 X82 0.69 X164 X236 0.23 X113 0.65 X52 X93 0.20 X76 0.77 X257 X237 0.23 X103 0.65 X20 X94 0.20 X90 0.80 X184 X238 0.21 X100 0.73 X14 X95 0.25 X15 0.77 X141 X239 0.15 X205 0.73 X257 X96 0.22 X93 0.72 X266 X240 0.21 X248 0.67 X13 X97 0.24 X118 0.74 X127 X241 0.16 X198 0.67 X189 X98 0.16 X99 0.69 X259 X242 0.08 X123 0.70 X114 X99 0.11 X118 0.73 X259 X243 0.18 X32 0.67 X15 X100 0.15 X103 0.77 X257 X244 0.19 X169 0.63 X20 X101 0.19 X99 0.70 X165 X245 0.22 X267 0.63 X40 X102 0.24 X250 0.70 X15 X246 0.24 X45 0.64 X88 X103 0.15 X100 0.75 X257 X247 0.23 X53 0.77 X94 X104 0.18 X103 0.74 X81 X248 0.14 X123 0.66 X280 X105 0.24 X189 0.73 X108 X249 0.13 X260 0.69 X94 X106 0.24 X116 0.74 X247 X250 0.19 X141 0.68 X13 X107 0.15 X29 0.73 X47 X251 0.08 X150 0.76 X189 X108 0.11 X285 0.73 X105 X252 0.08 X137 0.70 X97 X109 0.11 X234 0.69 X95 X253 0.06 X166 0.66 X106 X110 0.21 X56 0.63 X105 X254 0.18 X134 0.82 X205 X111 0.07 X112 0.63 X269 X255 0.27 X135 0.75 X227 X112 0.07 X111 0.66 X268 X256 0.16 X120 0.72 X114 X113 0.22 X7 0.75 X52 X257 0.15 X184 0.83 X205 X114 0.18 X118 0.77 X123 X258 0.10 X123 0.73 X107 X115 0.09 X201 0.66 X9 X259 0.20 X131 0.75 X276 X116 0.11 X118 0.70 X247 X260 0.13 X249 0.72 X94 X117 0.15 X78 0.73 X278 X261 0.17 X229 0.63 X15 X118 0.11 X99 0.76 X46 X262 0.08 X149 0.73 X148 X119 0.18 X187 0.73 X221 X263 0.16 X144 0.71 X9 X120 0.16 X256 0.66 X175 X264 0.23 X248 0.69 X106 X121 0.13 X172 0.69 X97 X265 0.13 X69 0.65 X15 X122 0.23 X143 0.66 X52 X266 0.13 X168 0.73 X15 X123 0.08 X242 0.77 X114 X267 0.20 X266 0.67 X96 X124 0.20 X157 0.66 X7 X268 0.20 X59 0.73 X106 X125 0.19 X170 0.75 X7 X269 0.19 X161 0.77 X89 X126 0.05 X143 0.66 X164 X270 0.13 X162 0.69 X189 X127 0.16 X162 0.74 X97 X271 0.17 X131 0.67 X99 X128 0.26 X9 0.66 X173 X272 0.13 X131 0.69 X114 X129 0.08 X191 0.73 X231 X273 0.29 X50 0.72 X96 X130 0.03 X133 0.73 X93 X274 0.13 X266 0.73 X114 X131 0.13 X272 0.71 X107 X275 0.25 X60 0.65 X189 X132 0.15 X222 0.66 X114 X276 0.23 X280 0.77 X161 X133 0.03 X130 0.72 X93 X277 0.17 X272 0.64 X193 X134 0.12 X212 0.69 X205 X278 0.16 X193 0.73 X117 X135 0.24 X269 0.66 X239 X279 0.14 X5 0.64 X280 X136 0.18 X168 0.68 X177 X280 0.23 X276 0.70 X150 X137 0.08 X252 0.70 X15 X281 0.21 X131 0.68 X13 X138 0.21 X250 0.70 X8 X282 0.28 X286 0.72 X163 X139 0.24 X122 0.73 X160 X283 0.21 X119 0.67 X89 X140 0.23 X46 0.70 X118 X284 0.13 X150 0.79 X173 X141 0.19 X250 0.77 X95 X285 0.11 X108 0.69 X107 X142 0.16 X260 0.72 X276 X286 0.22 X283 0.70 X88 X143 0.05 X126 0.66 X88 X287 0.30 X280 0.67 X14 X144 0.16 X263 0.72 X15 X288 0.15 X285 0.73 X107