病毒介导的GFP-ATG8在小麦上的表达和在自噬活性监测上的应用

胡蕊洁, 杨向芸, 贾磊, 李玉如, 项月, 岳洁瑜, 王华忠

Virus-mediated expression of GFP-ATG8 for autophagy monitoring in wheat

HU Rui-Jie, YANG Xiang-Yun, JIA Lei, LI Yu-Ru, XIANG Yue, YUE Jie-Yu, WANG Hua-Zhong

图8 融合蛋白GFP-TaATG8a在小麦根细胞中的定位 将过表达GFP-TaATG8a小麦植株的根部剪下, 置于蒸馏水中并保存于黑暗条件下进行饥饿处理, 蒸馏水中添加终浓度1 μmol L-1的刀豆素A (Starvation/ConA)或1%溶剂DMSO (Starvation/-), 同时设置不经饥饿和刀豆素A处理的非离体根组织对照(-/-)。于处理后24 h在共聚焦显微镜下观察根细胞中的GFP荧光并照相。标尺为75 μm。

Fig. 8 Localization of GFP-TaATG8a proteins in the root cells of wheat Wheat roots expressing GFP or GFP-TaATG8a were detached and treated with starvation (Starvation/-) or starvation plus Concanamycin A (ConA) (Starvation/ConA) by keeping them in distilled water containing 1 μmol L-1 ConA or 1% solvent DMSO under dark conditions. Roots of intact plants that were not treated with starvation and ConA (-/-) were used as controls. GFP fluorescence in root cells was observed under a laser scanning confocal microscope at 24 hours after starvation treatment. Bar: 75 μm.