玉米小籽粒突变体mn-Mu的基因克隆与转录组分析

丁孟丽, 王茹茵, 施栋晟, 李莹博, 雷洁, 陈洪宇, 申清文, 王桂凤

Map-based cloning and transcriptomic analysis of a maize miniature kernel mutant mn-Mu

DING Meng-Li, WANG Ru-Yin, SHI Dong-Sheng, LI Ying-Bo, LEI Jie, CHEN Hong-Yu, SHEN Qing-Wen, WANG Gui-Feng

图4 玉米mn-Mu的基因定位与克隆 A: 玉米mn-Mu的图位克隆。n: 定位所用的群体数目, 与InDel标记相对应的数值为交换籽粒数目; B: Mn1基因结构示意图。三角符号代表mn-Mu中转座子插入位置; C: Mn1基因DNA全长扩增。M: DNA分子量标记; WT: 野生型; Z58: 自交系郑58对照。

Fig. 4 Map-based cloning of mn-Mu gene in maize A: gene mapping of mn-Mu. n: the number of individuals for gene mapping, the number under each InDel marker indicates recombinants in the population. B: schematic diagram of Mn1 gene with the indicated mutator insertion site in mn-Mu by a triangle. C: PCR amplification of Mn1 gene using genomic DNA to detect the mutator insertion in mn-Mu mutant. M: marker; WT: wild type; Z58: the inbred line Zheng 58 as a control.