泛素受体蛋白OsDSK2b负向调控水稻叶瘟和渗透胁迫抗性

丁杰荣, 马雅美, 潘发枝, 江立群, 黄文洁, 孙炳蕊, 张静, 吕树伟, 毛兴学, 于航, 李晨, 刘清

Ubiquitin receptor protein OsDSK2b plays a negative role in rice leaf blast resistance and osmotic stress tolerance

DING Jie-Rong, MA Ya-Mei, PAN Fa-Zhi, JIANG Li-Qun, HUANG Wen-Jie, SUN Bing-Rui, ZHANG Jing, LYU Shu-Wei, MAO Xing-Xue, YU Hang, LI Chen, LIU Qing

图1 OsDSK2b的表达受多种胁迫影响以及OsDSK2b在日本晴中的时空表达模式 A: OsDSK2b在稻瘟病分离株Guy11、20% PEG-6000、150 mmol L-1 NaCl和100 μmol L-1ABA处理前后的相对表达量; B: OsDSK2b在日本晴不同组织(2周龄幼苗、幼根、茎节、倒二叶、旗叶、孕穗期根、幼穗和抽穗期穗)中的相对表达量; C: OsDSK2b蛋白在水稻原生质体中的亚细胞定位(标尺为10 μm)。数据均为3个生物学重复的平均值(±标准差) (Dunnett’s test, *: P < 0.05, **: P < 0.01)

Fig. 1 Relative expression pattern of OsDSK2b gene affected by various stresses and the spatio-temporal expression model of OsDSK2b in Nipponbare A: the relative expression levels of OsDSK2b before and after Guy11, 20% PEG-6000, 150?mmol L-1 NaCl, and 100 μmol L-1ABA treatments. B: the relative expression levels of OsDSK2b in different tissues of Nipponbare (two-week-old seedlings, young roots, node, second leaf, flag leaves, root of booting stage, young panicle, and panicle of heading stage). C: the subcellular localization of OsDSK2b in rice protoplast (Bar: 10 μm). Values are means (± SDs) from three biological replicates (by Dunnett’s test, *: P < 0.05, **: P < 0.01).