泛素受体蛋白OsDSK2b负向调控水稻叶瘟和渗透胁迫抗性

丁杰荣, 马雅美, 潘发枝, 江立群, 黄文洁, 孙炳蕊, 张静, 吕树伟, 毛兴学, 于航, 李晨, 刘清

Ubiquitin receptor protein OsDSK2b plays a negative role in rice leaf blast resistance and osmotic stress tolerance

DING Jie-Rong, MA Ya-Mei, PAN Fa-Zhi, JIANG Li-Qun, HUANG Wen-Jie, SUN Bing-Rui, ZHANG Jing, LYU Shu-Wei, MAO Xing-Xue, YU Hang, LI Chen, LIU Qing

图2 敲除OsDSK2b增强水稻对稻瘟病的抗性 A: OsDSK2b的结构和各敲除株系的靶序列突变情况。外显子和内含子分别用黑色矩形和黑线表示, 红色字体表示CRISPR载体构建的靶序列, 蓝色字体表示插入的碱基, 省略号表示缺失的碱基; B: 打孔接种GD08-T13菌株7 d后, 野生型日本晴和OsDSK2b敲除株系的表型图; C: 打孔接种GD08-T13菌株7 d后, OsDSK2b敲除株系和野生型日本晴的病斑面积统计图; D: 离体接种Guy11菌株7 d后, 野生型日本晴和OsDSK2b敲除株系的表型图; E: 接种Guy11菌株7 d后, OsDSK2b敲除株系和野生型日本晴的病斑面积统计图。 数据为3个生物学重复的平均值(±标准差) (Dunnett’s测验, *: P < 0.05, **: P < 0.01)。

Fig. 2 Knocking out of OsDSK2b enhances rice resistance to blast disease A: the gene structure of OsDSK2b and mutation of target sequence in OsDSK2b knockout strains. Exon and intron are represented by the blank rectangle black line. Red font represents the target sequence in CRISPR vector construction, blue fonts represent the inserted bases, and ellipsis represent the missing bases; B: the phenotype of wild-type Nipponbare and OsDSK2b knockout plants 7 d after inoculation with GD08-T13 using the punch method; C: the lesion sizes of wild-type Nipponbare and OsDSK2b knockout plants 7 d after inoculation with GD08-T13 using the punch method; D: the phenotype of wild-type Nipponbare and OsDSK2b knockout plants 7 d after inoculation with Guy11 in vivo; E: The lesion sizes of wild-type Nipponbare and OsDSK2b knockout plants 7 d after inoculation with Guy11 in vivo. Values are means (± SDs) from three biological replicates (Dunnett’s test, *: P < 0.05, **: P < 0.01).