泛素受体蛋白OsDSK2b负向调控水稻叶瘟和渗透胁迫抗性

丁杰荣, 马雅美, 潘发枝, 江立群, 黄文洁, 孙炳蕊, 张静, 吕树伟, 毛兴学, 于航, 李晨, 刘清

Ubiquitin receptor protein OsDSK2b plays a negative role in rice leaf blast resistance and osmotic stress tolerance

DING Jie-Rong, MA Ya-Mei, PAN Fa-Zhi, JIANG Li-Qun, HUANG Wen-Jie, SUN Bing-Rui, ZHANG Jing, LYU Shu-Wei, MAO Xing-Xue, YU Hang, LI Chen, LIU Qing

图4 敲除OsDSK2b增强水稻对渗透胁迫的抗性 A: 20% PEG-6000处理前与处理3?d再复水生长5?d后, 野生型日本晴和OsDSK2b敲除株系的表型图; B: 20% PEG-6000处理3?d再复水生长5 d后, 野生型日本晴和OsDSK2b株高统计图; C: 20% PEG-6000处理前与处理5?d再复水生长5 d后, 野生型日本晴和OsDSK2b敲除株系的表型图; D: 20% PEG-6000处理5 d再复水生长5 d后, 野生型日本晴和OsDSK2b的存活率统计图; E: 20% PEG-6000处理前后, 野生型日本晴和OsDSK2b的离子渗透率统计图; F: 野生型日本晴与OsDSK2b敲除株系地上部在0、30、60、90、120、180、240和300 min的离体失水率统计图。数据均为3个生物学重复的平均值(±标准差) (Dunnett’s测验, *: P < 0.05; **: P < 0.01)。

Fig. 4 Knocking out OsDSK2b increases the tolerance to osmotic stress in rice A: the phenotype of wild-type Nipponbare and OsDSK2b knockout plants under normal condition or 3 d 20% PEG-6000 treatment and 5 d recovery; B: the plant height of wild-type Nipponbare and OsDSK2b knockout plants under normal condition or 3 d 20% PEG-6000 treatment and 5 d recovery; C: the phenotype of wild-type Nipponbare and OsDSK2b knockout plants under normal condition or 5 d 20% PEG-6000 treatment and 5 d recovery; D: the survival rates of wild-type Nipponbare and OsDSK2b knockout plants under normal condition or 5 d 20% PEG-6000 treatment and 5 d recovery; E: the ion leakage of wild-type Nipponbare and OsDSK2b knockout plants before and after osmotic stress treatment; F: the water loss rates of seedlings of wild-type Nipponbare and OsDSK2b knockout plants at 0, 30, 60, 90, 120, 180, 240, and 300?min in vitro. Values are means (± SDs) of three biological replicates (Dunnett’s test, *: P < 0.05; **: P < 0.01).