转录组结合区域关联分析挖掘油菜含油量积累的候选基因

曹松, 姚敏, 任睿, 贾元, 向星汝, 李文, 何昕, 刘忠松, 官春云, 钱论文, 熊兴华

A combination of genome-wide association and transcriptome analysis reveal candidate genes affecting seed oil accumulation in Brassica napus

CAO Song, YAO Min, REN Rui, JIA Yuan, XIANG Xing-Ru, LI Wen, HE Xin, LIU Zhong-Song, GUAN Chun-Yun, QIAN Lun-Wen, XIONG Xing-Hua

图4 50个重测序材料中单体型(1,944,128~1,994,025 bp)区域含油量关联分析 (a) 单体型(1,944,128~1,994,025 bp; R2=0.99)区域的含油量关联分析。蓝色实线表示全基因组显著性的阈值P值为1.0×10-4。(b)和(c) 3个SNP (Chr.A10: 1,969,139; Chr.A10: 1,969,444; Chr.A10: 1,969,452, P=1.35×10-5)与含油量显著相关, 并定位在BnOBAP1a-A10基因启动子区域。热图显示这些SNPs存在强的连锁不平衡。在BnOBAP1a-A10单倍型区检测到2个单倍型等位基因。(d) 比较分析2个单体型等位基因对应材料的含油量。单体型等位基因在群体中的频率大于0.01将被用于此分析。箱型图显示BnOBAP1a-A10_Hap1等位基因对应的材料含油量显著高于BnOBAP1a-A10_Hap2对应的材料含油量。*、**、***分别表示在0.05、0.01和0.001概率水平差异显著。

Fig. 4 Correlation analysis of oil content in haplotype (1,944,128-1,994,025 bp) regions of 50 resequencing materials (a) Haplotype (1,944,128-1,994,025 bp; R2=0.99) oil content correlation analysis. The solid blue line indicates a threshold P-value of 1.0×10-4 for genome-wide significance. (b)-(c) three SNPs (Chr. A10: 1,969,139; Chr. A10: 1,969,444; Chr. A10: 1,969,452, P = 1.35×10-5) was significantly correlated with oil content and was localized in the promoter region of BnOBAP1a-A10 gene. Heat maps show a strong linkage imbalance in these SNPs. Two haplotype alleles were detected in the BnOBAP1a-A10 haplotype region. (d) Comparative analysis of the oil content of the materials corresponding to the two haplotype alleles. Haplotype alleles with frequencies greater than 0.01 in the population will be used for this analysis. The box pattern shows that the material corresponding to the BnOBAP1a-A10_Hap1 allele has a higher oil content than that of BnOBAP1a-A10_Hap2. *, **, and *** mean significant difference at the 0.05, 0.01, and 0.001 probability levels, respectively.