东北春播区糜子核心种质及其DNA分子身份证构建

丁艺冰, 辛旭霞, 冯智尊, 曹越, 郭娟, Dipak K SANTRA, 王瑞云, 陈喜明

Core germplasm and DNA molecular identity card of proso millet in Northeast Spring sowing region in China

DING Yi-Bing, XIN Xu-Xia, FENG Zhi-Zun, CAO Yue, GUO Juan, Dipak K SANTRA, WANG Rui-Yun, CHEN Xi-Ming

表2 构建核心种质的30对SSR引物

Table 2 30 SSR primer pairs for construction of the core germplasm

引物 Primer name	正向引物序列 Forward sequence (5′-3′)	反向引物序列 Reverse sequence (5′-3′)	退火温度 T_m (℃)	重复碱基 Repeat motif
RYW2	TTAGGGCTCTCCTGCATCC	CAGCGAGTTCACCGTCAAG	56.8	(CGAAGC)5
RYW3	GGAGGCGTGACAATAAAAC	GGCGTGAGGTGTTGTTTTT	52.5	(CTGCAA)5
RYW5	GACGATGCTCTTGACCTTGT	CACCGTGAAATGTCTCTGCT	55.0	(CCTTT)5
RYW6	AGCCGATTTGCTGTGGAGT	CTGCCTCCGATGAGTTGGT	57.4	(ACACC)5
RYW8	GGGTCAGAGAATACACAGCG	GTAGGGAAGGAGAAGTGGGT	55.9	(AATAG)5
RYW12	ACCATCCCAGCACAAACCA	TGCCTGAAGGAGAAGAGCG	57.5	(AGCT)5
RYW16	ATCTCCTCCGCCTTCTAACCC	TGGCAATGGTCGTACAAACT	56.7	(GAGC)5
RYW17	TCAGCTACTTCGAACGGC	GGATCATGCGATACATTTGG	53.0	(TTTC)5
RYW20	ACCTCTTGCCGCACACTAC	TTCTACATCCCCGAACCAC	56.2	(TTGG)6
RYW28	CCAAGGCTGAGCAGAAAGAT	ACAAGGTGAAACCCGAAGC	55.4	(AGGC)5
RYW29	CTTGATTTCTCACGCACCG	TGTCCAGCAGTAGTCGTTCCT	56.2	(GCAG)5
RYW30	TAGCCTTCTTTGCCACCACT	GCCCGTGATGATATTCGAC	54.9	(TTTC)5
RYW40	TGCTCTTCGGCTCTTCTCC	ATCAGCTCATCGTGACCCC	57.4	(CAGC)6
RYW42	AGACACCCTGGGCAACATC	CTGGACTGGGCTTCGTTCT	57.8	(GGCT)5
RYW43	GGAGATGCTTGCTTGGTTG	CAGGAATCGCAAGGAACAG	54.0	(GGAG)5
RYW47	TTGTTTTTGCTGCTGCCTC	TGCTGGACTTCTTTTTGCC	53.7	(GCCT)5
RYW50	CAAGGCAGATAGGGCAAGT	TCGTCTGCTGCTGGTTTGT	56.1	(GGAG)5
RYW52	AGTAGTCCTCCACCGCCAT	CTCTTCCTCGTTCTCGGCT	57.8	(TACC)5
RYW53	ATGCCTCCGATGTAGATGC	GCCGCCTTCTCTTCATTCT	54.9	(GAGG)5
RYW55	CTGGTGGTGGTAGTTAGCG	TTATGCCACCCACCGTAGC	56.9	(TAGC)5
RYW62	GTTTAGAGAGCAGGAGGCG	AGCCCTGTCCACCCTAATC	56.4	(GCTC)5
RYW67	GAAGGAAACGCACCAGAGT	TTGGGTTTGTGCTTGGAGT	54.9	(TGCG)5
RYW99	CGGAGTTCTTGGTGGCTT	GCGTTCGCCAAAGAGCAT	56.1	(CCA)5
RYW125	TTGACGACGACTGTGTGC	TGTTGGTGGAGTTGAGGAC	55.1	(GGC)5
RYW145	CTTTTTCTGCTGCTCCCT	TGATGCCATACCCAACTG	52.2	(GAC)6
RYW146	TGATGCTTCTTGGGTTCG	CGCCGTCCACTTCTGTAT	53.6	(GCG)6
RYW149	CAGGACTTGGGTGATTGC	GAGCGGAGGAGGAAACTA	53.7	(AGC)7
RYW156	TTTACAACCCTTCCCGCC	AGGACTTTCCGCCTCTACCC	57.5	(CCG)5
RYW158	GGTAGGGTTCAAGGTGGTT	CAGGCAATCTCTTCAGGC	54.0	(CCG)5
RYW164	AGACAGCCATTCAACCACG	CCATCTCCTCATCCACCA	54.6	(GA)6