基于转录组分析鉴定甘蓝型油菜开花候选基因以及BnaCOR27功能验证

王晨, 贺丹, 姚敏, 邱萍, 何昕, 熊兴华, 康雷, 刘忠松, 钱论文

Transcriptome-based identification of flowering candidate genes and functional characterization of BnaCOR27 in Brassica napus

WANG Chen, HE Dan, YAO Min, QIU Ping, HE Xin, XIONG Xing-Hua, KANG Lei, LIU Zhong-Song, QIAN Lun-Wen

图6 转基因植株靶点突变序列分析及开花表型 A: BnaCOR27在A09染色体的敲除载体以及靶点突变情况示意图; B: BnaCOR27在C09染色体的敲除载体以及靶点突变情况示意图; C: 敲除突变体植株CAS:BnaCOR27-T3及对照植株ZS6开花时间表型图片; D: 敲除突变株系CAS:BnaCOR27-T3及对照植株ZS6开花时间柱状图; **: P < 0.01。在A09染色体上出现大片段缺失, C09染色体上插入单碱基。敲除突变体植株CAS:BnaCOR27-T3相较于对照植株ZS6开花时间提前约6.54 d。ZS6: 中双6号。标尺为5 cm。

Fig. 6 Target site mutations and flowering phenotype of transgenic plants A: schematic diagram of BnaCOR27 knockout vector and target mutation on A09 chromosome; B: schematic diagram of BnaCOR27 knockout vector and target mutation on A09 chromosome; C: knockout mutant CAS:BnaCOR27-T3 and control plant ZS6 flowering time phenotype picture; D: knockout mutant lines CAS:BnaCOR27-T3 and control lines ZS6 flowering time bar graph; **: P < 0.01. There is a large deletion on chromosome A09, and a single base is inserted on chromosome C09. Knockout mutant plant CAS:BnaCOR27-T3 blooms about 6.54 days earlier than the control plant ZS6. ZS6: Zhongshuang 6. Bar = 5 cm.