烟草脂类合成关键基因NtLPAT的功能验证

吉白璐, 孙艺文, 刘万峰, 钱亚新, 蒋彩虹, 耿锐梅, 刘旦, 程立锐, 杨爱国, 黄立钰, 李晓旭, 蒲文宣, 高军平, 张强, 文柳璎

Functional verification of the key gene NtLPAT involved in lipid biosynthesis in tobacco

JI Bai-Lu, SUN Yi-Wen, LIU Wan-Feng, QIAN Ya-Xin, JIANG Cai-Hong, GENG Rui-Mei, LIU Dan, CHENG Li-Rui, YANG Ai-Guo, HUANG Li-Yu, LI Xiao-Xu, PU Wen-Xuan, GAO Jun-Ping, ZHANG Qiang, WEN Liu-Ying

图1 NtLPAT基因序列对比以及表达模式分析 图A中, M为DL 2000 marker, 1为阴性对照, 2为目的条带。图B为烟草、马铃薯、辣椒与番茄LPAT序列的比对。图C为NtLPAT在烟草不同组织中的表达量, Root: 地下部; Shoot: 地上部; Shoot apex: 苗端。图D为NtLPAT响应黑胫病的表达模式, 0、6、12、24 hpi表示接菌后0、6、12、24 h, R表示烟草抗黑胫品种BH1000-1, S表示感黑胫烟草品种小黄金1025。图E为NtLPAT响应青枯病表达模式, 3、9、24 hpi表示接菌后3、9、24 h, R表示烟草抗青枯品种反帝三号-丙, S表示烟草感青枯品种红花大金元, CK表示未接菌对照, RS表示接种青枯菌。*、**和***分别表示在0.05、0.01和0.001水平差异显著。

Fig. 1 Sequence alignment and expression pattern analysis of the NtLPAT gene In Fig. A, M represents the DL 2000 marker, lane 1 is the negative control, and lane 2 is the target band. Fig. B shows the alignment of LPAT sequences from tobacco, potato, pepper, and tomato. Fig. C illustrates the expression levels of NtLPAT in different tobacco tissues, Root: underground part; Shoot: aboveground part; Shoot apex: shoot tip. Fig. D illustrates the expression pattern of NtLPAT in response to blackleg disease, 0, 6, 12, and 24 hpi represent 0, 6, 12, and 24 hours post-inoculation, R denotes the black shank-resistant tobacco variety BH1000-1, and S denotes the black shank-susceptible tobacco variety Xiaohuangjin 1025. Fig. E illustrates the expression pattern of NtLPAT in response to Ralstonia solanacearum infection, 3, 9, and 24 hpi represent 3, 9, and 24 hours post-inoculation, R denotes the bacterial wilt-resistant tobacco variety Fandi 3-Bing, S denotes the bacterial wilt-susceptible tobacco variety Honghuadajinyuan, CK represents the non-inoculated control, and RS represents the inoculation with Ralstonia solanacearum. *, **, and *** indicate significant differences at the 0.05, 0.01, and 0.001 levels, respectively.