V型细胞质雄性不育小麦线粒体蛋白质的研究

作物学报 ›› 2001, Vol. 27 ›› Issue (02): 212-215.

V型细胞质雄性不育小麦线粒体蛋白质的研究

杨毅;滕晓月;阎隆飞

中国农业大学生物学院，北京 100094

收稿日期:1999-11-02 修回日期:2000-06-17 出版日期:2001-03-12 网络出版日期:2001-03-12
通讯作者: 杨毅

Studies on Mitochondrial Proteins of CMS-V in Wheat

Yang Yi;Teng Xiaoyue;Yan Longfei

Received:1999-11-02 Revised:2000-06-17 Published:2001-03-12 Published online:2001-03-12
Contact: Yang Yi

摘要/Abstract

摘要： 本实验以V型细胞质雄性不育小麦幼穗为材料，用蔗糖衬垫法和两相法成功地分离纯化了小麦幼穗线粒体，并用SDS-PAGE电泳方法结合高灵敏度的银染技术对其线粒体蛋白质进行了比较分析，发现雄性不育系与相应保持系之间有明显差异，不育系(V1A和V2A)均缺少一个分子量为14kD的蛋白质。线粒体与V型细胞质雄性不育的产生可能存在着某

关键词: V型细胞质雄性不育, 线粒体蛋白质, 梯度SDS-PAGE

Abstract: Two CMS-V lines of wheat(with the cytoplasm of Ae. ventricosa), V1A and V2A were used in this investigation. The mitochondria was prepared and purified successfully from wheat young ears via sucrose cushion and two-phase method. Differences of mitochondria polypeptides have been found between male s

Key words: Wheat, CMS-V, Mitochondrial polypeptides, Gradient SDS-PAGE,

中图分类号:

S512

杨毅;滕晓月;阎隆飞. V型细胞质雄性不育小麦线粒体蛋白质的研究[J]. 作物学报, 2001, 27(02): 212-215.

Yang Yi;Teng Xiaoyue;Yan Longfei. Studies on Mitochondrial Proteins of CMS-V in Wheat[J]. Acta Agron Sin, 2001, 27(02): 212-215.

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