大豆种子特异性启动子的克隆及序列分析

作物学报 ›› 2005, Vol. 31 ›› Issue (01): 11-17.

大豆种子特异性启动子的克隆及序列分析

财音青格乐;李明春;蔡易;陶然;邢来君

南开大学生命科学学院微生物学系，天津300071

收稿日期:2003-10-28 修回日期:2004-02-15 出版日期:2005-01-12 网络出版日期:2005-01-12
通讯作者: 邢来君

Isolation and Sequencing Analysis of the Seed-specific Promoter from Soybean

CAI-YIN Qing-Ge-Le; LI Ming-Chun; CAI Yi;TAO Ran ; XING Lai-Jun

Department of Microbiology, College of Life Science, Nanakai University, Tianjin 300071

Received:2003-10-28 Revised:2004-02-15 Published:2005-01-12 Published online:2005-01-12
Contact: XING Lai-Jun

摘要/Abstract

摘要： 通过PCR技术从大豆品种吉林43基因组DNA中分离到大豆β-伴球蛋白α-亚基基因启动子片段（BCSP489），对此片段采用TAIL PCR技术进一步延伸，获得了启动子片段BCSP666。序列分析表明，在启动子片段BCSP666中含有多种种子特异性启动子所特有的序列元件，如A/T富含序列元件、RY重复序列元件、AGCCCA序列元件、TACACAT序列元件、A

关键词: 种子特异性启动子, 序列元件, TAILPCR

Abstract: The low level of foreign genes’ expression in transgenic plants is a key factor that limits plant genetic engineering. Because of the critical regulatory activity of the promoters on gene transcription, they are studied extensively to improve the efficiency of plant transgenic system. The constitut

Key words: Seed-specific promoter, Motif, TAIL PCR

中图分类号:

S565

财音青格乐, 李明春, 蔡易, 陶然, 邢来君. 大豆种子特异性启动子的克隆及序列分析[J]. 作物学报, 2005, 31(01): 11-17.

CAI-YIN Qing-Ge-Le, LI Ming-Chun, CAI Yi, TAO Ran , XING Lai-Jun. Isolation and Sequencing Analysis of the Seed-specific Promoter from Soybean[J]. Acta Agronomica Sinica, 2005, 31(01): 11-17.

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