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作物学报 ›› 2026, Vol. 52 ›› Issue (10): 2971-2983.doi: 10.3724/SP.J.1006.2026.64037

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

PaZFP36过表达提高狼尾草的光合效率及生物量积累

郑美1(), 李萍1,2(), 李翠1(), 侯新村1, 赵春桥1, 赵雪铮1, 张乃文1, 范希峰1,*(), 郭强1,*()   

  1. 1 北京市农林科学院草业花卉与生态景观研究所, 北京 100097
    2 甘肃农业大学草业学院 / 草业生态系统教育部重点实验室 / 甘肃省草业工程实验室 / 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070
  • 收稿日期:2026-03-24 接受日期:2026-07-15 出版日期:2026-10-12 网络出版日期:2026-07-22
  • 通讯作者: 范希峰, E-mail: fanxifeng@baafs.net.cn;郭强, E-mail: guoqiang@baafs.net.cn
  • 作者简介:郑美, E-mail: zhengmei@baafs.net.cn;
    李萍, E-mail: liping@baafs.net.cn;
    李翠, E-mail: licui@baafs.net.cn** 同等贡献

Overexpression of PaZFP36 enhances photosynthetic efficiency and biomass accumulation in Pennisetum alopecuroides

Zheng Mei1(), Li Ping1,2(), Li Cui1(), Hou Xin-Cun1, Zhao Chun-Qiao1, Zhao Xue-Zheng1, Zhang Nai-Wen1, Fan Xi-Feng1,*(), Guo Qiang1,*()   

  1. 1 Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
    2 College of Pratacultural Science, Gansu Agricultural University / Key Laboratory of Grassland Ecosystem, Ministry of Education / Pratacultural Engineering Laboratory of Gansu Province / Sino-US Centers for Grazing Land Ecosystem Sustainability, Lanzhou 730070, Gansu, China
  • Received:2026-03-24 Accepted:2026-07-15 Published:2026-10-12 Published online:2026-07-22
  • Contact: Fan Xi-Feng, E-mail: fanxifeng@baafs.net.cn;Guo Qiang, E-mail: guoqiang@baafs.net.cn
  • About author:** Contributed equally to this work

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摘要：

C2H2型锌指蛋白在植物生长发育和逆境响应中发挥重要调控作用。本研究基于前期冷胁迫转录组数据, 从‘丽秋’狼尾草(Pennisetum alopecuroides cv. ‘Liqiu’)中克隆获得C2H2型锌指蛋白编码基因PaZFP36, 对其进行生物信息学分析, 并构建PaZFP36过表达载体, 获得转基因拟南芥(Arabidopsis thaliana)和‘丽秋’狼尾草, 通过盆栽试验对转基因株系的光合特性及成熟期农艺性状进行观察和系统分析。结果表明, PaZFP36蛋白分子量为24.65 kD, 等电点为8.49, 含有2个典型的C2H2锌指结构域。在拟南芥和‘丽秋’狼尾草中过表达PaZFP36均显著提高了净光合速率(Pn)、最大光化学效率(Fv/Fm)和光合性能指数(PIabs), 而胞间CO2浓度(Ci)显著降低, 表明PaZFP36通过提升叶肉细胞CO2固定能力、优化PSII反应中心性能和电子传递链效率, 协同增强光合效率。与野生型相比, 拟南芥及‘丽秋’狼尾草过表达PaZFP36株系的株高、鲜重、干重、主根长均显著增加; 转基因拟南芥的荚果长、主茎荚果数及转基因‘丽秋’狼尾草的分蘖数也显著提高。相关性分析显示, 净光合速率与干重呈显著正相关。因此, 过表达PaZFP36可以提高‘丽秋’狼尾草光合效率, 并促进生物量积累和产量形成, 为培育抗逆和高产的狼尾草新品种提供了种质资源及理论依据。

关键词: 狼尾草, PaZFP36, 光合特性, 叶绿素含量, 产量

Abstract:

C2H2-type zinc finger proteins play crucial regulatory roles in plant growth, development, and stress responses. Based on previous transcriptome data under cold stress, a C2H2-type zinc finger protein gene, PaZFP36, was cloned from Pennisetum alopecuroides cv. ‘Liqiu’ in this study. Bioinformatic analysis was performed, and overexpression vectors were constructed and transformed into Arabidopsis thaliana and P. alopecuroides cv. ‘Liqiu’. Photosynthetic characteristics and agronomic traits at maturity were systematically analyzed through pot experiments. The results showed that the PaZFP36 protein has a molecular weight of 24.65 kD and an isoelectric point of 8.49 and contains two typical C2H2 zinc finger domains. Overexpression of PaZFP36 in both Arabidopsis and P. alopecuroides cv. ‘Liqiu’ significantly increased the net photosynthetic rate (Pn), maximum photochemical efficiency (Fv/Fm), and photosynthetic performance index (PIabs), while significantly decreasing the intercellular CO2 concentration (Ci). These results indicate that PaZFP36 synergistically enhances photosynthetic efficiency by improving mesophyll CO2 fixation capacity, optimizing PSII reaction center performance, and enhancing electron transport chain efficiency. Compared with wild-type plants, PaZFP36-overexpressing lines of both Arabidopsis and P. alopecuroides cv. ‘Liqiu’ exhibited significantly increased plant height, fresh weight, dry weight, and main root length. In addition, transgenic Arabidopsis showed significant increases in pod length and pod number per main stem, whereas transgenic P. alopecuroides cv. ‘Liqiu’ showed a significant increase in tiller number. Correlation analysis revealed a significant positive relationship between net photosynthetic rate and dry weight. In conclusion, overexpression of PaZFP36 enhances photosynthetic efficiency and promotes biomass accumulation and yield formation in P. alopecuroides cv. ‘Liqiu’, providing germplasm resources and a theoretical basis for breeding stress-tolerant and high-yielding Pennisetum varieties.

Key words: Pennisetum alopecuroides, PaZFP36, photosynthetic characteristics, chlorophyll content, yield

表1

本研究所用的引物"

引物名称
Primer name		 引物序列
Primer sequence (5′-3′)
PaZFP36-F ATGGTCGCCAGCTTCGAAGC
PaZFP36-R ATCCGACATCCTGCGCTTCT
qRT-PaZFP36-F GGTGTCGGTCTCCCTGTC
qRT-PaZFP36-R CTCTGCACCTCTTCCTCCTC
qRT-At-actin-F AGTCCACCCTTCATCTTGTTCTC
qRT-At-actin-R GTCAGCCAAAGTTCTTCCATCT
qRT-Pa-actin-F CTGAGCGGGAAATTGTGAGG
qRT-Pa-actin-R CATGGATGGCTGGAAGAGGA
pBI121-GUS-PaZFP36-F CGGGGGACTCTAGAGGATCCATGGTCGCCAGCTTCGAAG
pBI121-GUS-PaZFP36-R GACTGACCACCCGGGGATCCATCCGACATCCTGCGCTTC
PaZFP36-OE-F ATGTTACGTCCTGTAGAAAC
PaZFP36-OE-R GGATTCACCACTTGCAAAGT

图1

‘丽秋’狼尾草PaZFP36的遗传转化 A: 愈伤组织的培养; B: 愈伤组织培养后进行侵染; C: 抗性愈伤组织的诱导筛选; D: 抗性丛生芽分化; E-F: 抗性苗生根。"

图2

PaZFP36基因结构及其蛋白质理化性质 A: 狼尾草PaZFP36基因的克隆; B: PaZFP36基因结构示意图; C: 蛋白质二级结构; D: 蛋白质亲疏水性预测结果; E: 蛋白质三级结构。"

图3

不同物种的ZFP36蛋白进化树及蛋白多序列比对 A: 进化树分析; B: 不同物种ZFP36蛋白的多序列比对。Pa: 狼尾草; Zm: 玉米; Mf: 五节芒; Pv: 柳枝稷; Ob: 短药野生稻; Os: 水稻; At: 拟南芥。"

图4

PaZFP36过表达拟南芥的分子鉴定、生长表型及光合特性 A: 野生型(WT)与过表达PaZFP36转基因株系(OE-10、OE-11、OE-22、OE-23、OE-40、OE-61)中PaZFP36基因的相对表达量; B: 组织化学GUS染色; C: 4周龄(a)、5周龄(b)的表型图及叶片大小(c), 比例尺= 1 cm; D: 株高; E: 鲜重; F: 干重; G: 主根长; H: 荚果长; I: 主茎荚果数; J: 净光合速率; K: 气孔导度; L: 胞间CO2浓度; M: 叶绿素含量; N: 最大光化学效率(Fv/Fm); O: 光合性能指数(PIabs)。WT: 野生型拟南芥; OE-10和OE-61为过表达PaZFP36的转基因株系; 比例尺= 1 cm。所有数据以平均值±标准差表示, **和*分别表示与野生型相比在0.01和0.05水平差异显著。"

图5

PaZFP36过表达‘丽秋’狼尾草的分子鉴定、生长表型及光合特性 A: T0代阳性转化苗PCR鉴定; B: 野生型(WT)与各过表达转基因株系(OE-1、OE-5、OE-6、OE-7、OE-8、OE-9、OE-10)中PaZFP36基因的相对表达量; C: 野生型(WT)、PaZFP36-OE-8和PaZFP36-OE-9植株整体表型, 比例尺= 2 cm; D: 株高; E: 鲜重; F: 干重; G: 叶宽; H: 分蘖数; I: 主根长; J: 净光合速率; K: 气孔导度; L: 胞间CO2浓度; M: 叶绿素含量; N: 最大光化学效率; O: 光合性能指数。WT: 野生型‘丽秋’狼尾草; OE-8和OE-9为过表达PaZFP36的转基因株系。所有数据以平均值±标准差表示。**和*分别表示与野生型相比在0.01和0.05水平差异显著。"

图6

PaZFP36过表达拟南芥(A)和‘丽秋’狼尾草(B)的光合与生长指标间的相关性分析 Pn: 净光合速率; Gs: 气孔导度; Ci: 胞间CO2浓度; Chla+b: 叶绿素含量; Fv/Fm: 最大光化学效率; PIabs: 光合性能指数; PH: 株高; FW: 鲜重; DW: 干重; MRL: 主根长; PL: 荚果长; NPMS: 主茎荚果数; LW: 叶宽; TN: 分蘖数。红色代表正相关, 青绿色代表负相关, 颜色越深代表相关程度越大。*和**分别表示在0.05和0.01概率水平相关性显著。"

[1] Xu R X, Pu Z, Han S X, et al. Managing forage for grain: strategies and mechanisms for enhancing forage production to ensure feed grain security. J Integr Agric, 2025, 24: 2025-2034.
doi: 10.1016/j.jia.2024.11.017
[2] 景海春, 胡伟娟, 金京波, 等. 加快饲草智能育种科技创新的思考与建议. 中国科学院院刊, 2025, 40(2): 310-319.
Jing H C, Hu W J, Jin J B, et al. Accelerate innovation of forage intelligent breeding technology: reflection and suggestions. Bull Chin Acad Sci, 2025, 40(2): 310-319 (in Chinese with English abstract).
[3] 李净雅. 农业农村部《“十四五”全国饲草产业发展规划》解读. 农村实用技术, 2024(3): 14-15.
Li J Y. Interpretation of the national forage industry development plan in the 14th Five-Year Plan by the ministry of agriculture and rural affairs. Rural Pract Technol, 2024(3): 14-15 (in Chinese with English abstract).
[4] 陈永广, 姜慧新, 董博颖, 等. 不同狼尾草属牧草对德州驴的营养价值评定. 中国饲料, 2022(3): 137-141.
Chen Y G, Jiang H X, Dong B Y, et al. Nutritional value evaluation of different Pennisetum forages for Dezhou donkeys. China Feed, 2022(3): 137-141 (in Chinese with English abstract).
[5] 王丽宏, 李会彬, 孙鑫博, 等. 狼尾草野生种质资源的评价与分析. 中国农业科技导报, 2016, 18(3): 134-140.
doi: 10.13304/j.nykjdb.2015.722
Wang L H, Li H B, Sun X B, et al. Evaluation and analysis of wild germplasm resources of Pennisetum. J Agric Sci Technol, 2016, 18(3): 134-140 (in Chinese with English abstract).
[6] 李萍, 陈鑫, 赵春桥, 等. 高大禾草的栽培与综合利用研究进展. 植物学报, 2024, 59: 847-860.
doi: 10.11983/CBB23147
Li P, Chen X, Zhao C Q, et al. Research progress on cultivation and comprehensive utilization of tall grasses. Bull Bot, 2024, 59: 847-860 (in Chinese with English abstract).
[7] Yue Y S, Fan X F, Hu Y F, et al. In vitro induction and characterization of hexaploid Pennisetum×Advena, an ornamental grass. Plant Cell Tiss Organ Cult, 2020, 142: 221-228.
doi: 10.1007/s11240-020-01814-5
[8] Xu J, Liu C, Song Y, et al. Comparative analysis of the chloroplast genome for four Pennisetum species: molecular structure and phylogenetic relationships. Front Genet, 2021, 12: 687844.
doi: 10.3389/fgene.2021.687844
[9] Hayat K, Bundschuh J, Jan F, et al. Combating soil salinity with combining saline agriculture and phytomanagement with salt-accumulating plants. Crit Rev Environ Sci Technol, 2020, 50: 1085-1115.
doi: 10.1080/10643389.2019.1646087
[10] Guo Y D, Liu L Y, Yue Y S, et al. Development of SSR markers based on transcriptome sequencing and verification of their conservation across species of ornamental Pennisetum rich. (Poaceae). Agronomy, 2022, 12: 1683.
doi: 10.3390/agronomy12071683
[11] Yan H D, Sun M, Zhang Z R, et al. Pangenomic analysis identifies structural variation associated with heat tolerance in pearl millet. Nat Genet, 2023, 55: 507-518.
doi: 10.1038/s41588-023-01302-4 pmid: 36864101
[12] 景海春, 张景昱, 金京波, 等. 饲草作物基础生物学与生物育种. 中国科学基金, 2023, 37: 537-551.
Jing H C, Zhang J Y, Jin J B, et al. Fundamental biology and molecular breeding of forage crops. Bull Natl Nat Sci Found China, 2023, 37: 537-551 (in Chinese with English abstract).
[13] Ma L, Zhang Y C, Wen H Y, et al. Silencing of MsD14 resulted in enhanced forage biomass through increasing shoot branching in alfalfa (Medicago sativa L.). Plants, 2022, 11: 939.
doi: 10.3390/plants11070939
[14] Cen H F, Wang T T, Liu H Y, et al. Overexpression of MsASMT1 promotes plant growth and decreases flavonoids biosynthesis in transgenic alfalfa (Medicago sativa L.). Front Plant Sci, 2020, 11: 489.
doi: 10.3389/fpls.2020.00489
[15] Gao R M, Gruber M Y, Amyot L, et al. SPL13 regulates shoot branching and flowering time in Medicago sativa. Plant Mol Biol, 2018, 96: 119-133.
doi: 10.1007/s11103-017-0683-8
[16] Long Q Y, Huang Y X, Xie H P, et al. ABA insensitive 5 mediates abscisic acid regulation of plant photosynthesis by coordinating carbon and nitrogen assimilation. New Phytol, 2025, 248: 1903-1919.
doi: 10.1111/nph.v248.4
[17] 钟婵娟, 彭伟业, 王冰, 等. 植物逆境响应相关的C2H2型锌指蛋白研究进展. 植物生理学报, 2020, 56: 2356-2366.
Zhong C J, Peng W Y, Wang B, et al. Advances of plant C2H2 zinc finger proteins in response to stresses. Plant Physiol J, 2020, 56: 2356-2366 (in Chinese with English abstract).
[18] Chen W, Zhu T, Shi Y N, et al. An antisense intragenic lncRNA SEAIRa mediates transcriptional and epigenetic repression of SERRATE in Arabidopsis. Proc Natl Acad Sci USA, 2023, 120: e2216062120.
[19] Zhang D, Ding X P, Wang Z J, et al. A C2H2 zinc finger protein, OsZOS2-19, modulates ABA sensitivity and cold response in rice. Plant Cell Physiol, 2025, 66: 753-765.
doi: 10.1093/pcp/pcaf018 pmid: 39916472
[20] Li M H, Zhu C H, Jiang C, et al. Diverse functions of C2H2 zinc-finger transcription factors in growth regulation and stress adaptation in woody and perennial horticultural plants. Hortic Plant J, 2026, 12: 1793-1809.
doi: 10.1016/j.hpj.2025.08.006
[21] 黄凯. 水稻C2H2型转录因子OsZFP151在低温胁迫中的功能研究. 吉林大学硕士学位论文, 吉林长春, 2017.
Huang K. Functional Analysis of C2H2 Transcription Factor OsZFP151 in Rice (Oryza sativa L.) under Low Temperature Stress. MS Thesis of Jilin University, Changchun, Jilin, China, 2017 (in Chinese with English abstract).
[22] Gao Y H, Li Z, Yang C Y, et al. Pseudomonas syringae activates ZAT18 to inhibit salicylic acid accumulation by repressing EDS1 transcription for bacterial infection. New Phytol, 2022, 233: 1274-1288.
doi: 10.1111/nph.v233.3
[23] Yin M Z, Wang Y P, Zhang L H, et al. The Arabidopsis Cys2/His2 zinc finger transcription factor ZAT18 is a positive regulator of plant tolerance to drought stress. J Exp Bot, 2017, 68: 2991-3005.
doi: 10.1093/jxb/erx157
[24] Li Y, Chu Z N, Luo J Y, et al. The C2H2 zinc-finger protein SlZF3 regulates AsA synthesis and salt tolerance by interacting with CSN5B. Plant Biotechnol J, 2018, 16: 1201-1213.
doi: 10.1111/pbi.2018.16.issue-6
[25] Lu J H, Pan C Y, Li X, et al. OBV (obscure vein), a C2H2 zinc finger transcription factor, positively regulates chloroplast development and bundle sheath extension formation in tomato (Solanum lycopersicum) leaf veins. Hortic Res, 2021, 8: 230.
doi: 10.1038/s41438-021-00659-z
[26] Luo J Y, Tang Y F, Chu Z N, et al. SlZF 3 regulates tomato plant height by directly repressing SlGA20ox4 in the gibberellic acid biosynthesis pathway. Hortic Res, 2023, 10: uhad025.
[27] Chen J Y, Zeng H Y, Yan F Y, et al. Identification of the WRKY gene family in Bergenia purpurascens and functional analysis of BpWRKY 13 under cold stress. Plant Physiol Biochem, 2025, 223: 109832.
doi: 10.1016/j.plaphy.2025.109832
[28] Baillo E H, Hanif M S, Guo Y H, et al. Genome-wide identification of WRKY transcription factor family members in sorghum (Sorghum bicolor (L.) moench). PLoS One, 2020, 15: e0236651.
[29] Xie J M, Chen Y R, Cai G J, et al. Tree Visualization By One Table (tvBOT): a web application for visualizing, modifying and annotating phylogenetic trees. Nucleic Acids Res, 2023, 51: W587-W592.
doi: 10.1093/nar/gkad359 pmid: 37144476
[30] Letunic I, Bork P. 20 years of the SMART protein domain annotation resource. Nucleic Acids Res, 2018, 46: D493-D496.
[31] Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J, 1998, 16: 735-743.
[32] Wang Z Y, Zhang L Y, Zhou B, et al. A single-MYB transcription factor GmMYB331 regulates seed oil accumulation and seed size/weight in soybean. J Integr Plant Biol, 2026, 68: 470-485.
doi: 10.1111/jipb.70101
[33] Hu Z R, Huang X B, Amombo E, et al. The ethylene responsive factor CdERF 1 from bermudagrass (Cynodon dactylon) positively regulates cold tolerance. Plant Sci, 2020, 294: 110432.
doi: 10.1016/j.plantsci.2020.110432
[34] Zhao L, Ma X M, Ma W X, et al. MsPYL6 and MsPYL9 improves drought tolerance by regulating stomata in alfalfa (Medicago sativa). Plant J, 2025, 122: e70265.
[35] Zhu S P, Nong J F, Luo G T, et al. Varied tolerance and different responses of five Citrus rootstocks to acid stress by principle component analysis and orthogonal analysis. Sci Hortic, 2021, 278: 109853.
doi: 10.1016/j.scienta.2020.109853
[36] Wu H Y, Liu Z Q, Zhang W F, et al. Does leaf lifespan affect the regulation of photosynthetic function during natural senescence of leaves in various sorghum cultivars? J Exp Bot, 2025, 76: 4071-4084.
doi: 10.1093/jxb/eraf195
[37] 贾双伟, 高英, 赵开军. 水稻OsLecRK基因家族表达模式分析. 作物学报, 2014, 40: 1174-1181.
doi: 10.3724/SP.J.1006.2014.01174
Jia S W, Gao Y, Zhao K J. Expression pattern analysis of OsLecRK gene family in rice. Acta Agron Sin, 2014, 40: 1174-1181 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2014.01174
[38] 黄周详, 李乙弘, 刘笑言, 等. 6-BA与烯效唑对前旱后湿胁迫下甘薯光合性能、抗逆生理及产量品质的影响. 作物学报, 2026, 52: 2521-2532.
doi: 10.3724/SP.J.1006.2026.54158
Huang Z X, Li Y H, Liu X Y, et al. Effects of 6-BA and uniconazole on photosynthetic performance, stress resistance physiology, yield and quality of sweetpotato under pre-drought and post-wetting stress. Acta Agron Sin, 2026, 52: 2521-2532 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2026.54158
[39] Li J X, Bai X M, Ran F, et al. Response mechanisms of Annual bluegrass (Poa annua) to cold, drought, combined stresses and recovery in morphology, photosynthesis, physiology and microstructure. Plant Physiol Biochem, 2024, 217: 109238.
doi: 10.1016/j.plaphy.2024.109238
[40] Li T, Guo J J, Zhang J H, et al. ZmDof08, a zinc finger transcription factor, plays critical roles in photosynthesis in maize. Plant Cell Rep, 2025, 44: 207.
doi: 10.1007/s00299-025-03591-x pmid: 40903564
[41] Zhuang Y M, Chen Y, Wang Q, et al. Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance. For Res, 2024, 4: e011.
[42] 王琪, 王陈玉, 郑烨, 等. 低温胁迫恢复过程中不同回温速度对油茶光系统活性的影响. 西南林业大学学报(自然科学), 2025, 45(3): 35-43.
Wang Q, Wang C Y, Zheng Y, et al. Effects of different rewarming rates on photosystem activity of Camellia oleifera during recovery from low-temperature stress. J Southwest For Univ (Nat Sci), 2025, 45(3): 35-43 (in Chinese with English abstract).
[43] Yang H Z, Yuan Y, Liu X Y, et al. Phytohormonal homeostasis, chloroplast stability, and heat shock transcription pathways related to the adaptability of creeping bentgrass species to heat stress. Protoplasma, 2025, 262: 649-665.
doi: 10.1007/s00709-024-02022-0 pmid: 39794516
[44] 汪华, 苏明, 吴佳瑞, 等. 宁南山区马铃薯叶片叶绿素荧光、物质积累与转运对磷素添加的响应. 生态学杂志, 2026, 45: 1880-1889.
Wang H, Su M, Wu J R, et al. Responses of chlorophyll fluorescence, matter accumulation and translocation in potato leaves to phosphorus addition in the mountainous area of southern Ningxia. Chin J Ecol, 2026, 45: 1880-1889 (in Chinese with English abstract).
[45] 马晨铭, 哈斯图亚, 常泓博, 等. 基于叶绿素荧光成像系统的马铃薯叶片叶绿素含量估测. 作物学报, 2026, 52: 2193-2206.
doi: 10.3724/SP.J.1006.2026.54157
Ma C M, Hasi T Y, Chang H B, et al. Estimation of chlorophyll content in potato leaves based on chlorophyll fluorescence imaging system. Acta Agron Sin, 2026, 52: 2193-2206 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2026.54157
[46] Yang Z, Zhang X, Zheng J T, et al. Substrate specificity and transport mechanism of the chloroplast dicarboxylate transporters DiT1 and DiT2. Plant Cell, 2026, 38: koag041.
[47] Chen L P, Yuan Y K, Wang P B, et al. A major latex protein, TaSTP, coordinates sugar-antioxidant synergy to enhance drought tolerance in wheat. Plant J, 2026, 125: e70753.
[48] Fu J B, Zhao Y Y, Min Y, et al. ERF026 balances the growth and drought stress response of Medicago sativa L. by regulating jasmonic acid metabolism. Plant Physiol, 2025, 199: kiaf606.
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