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作物学报 ›› 1986, Vol. 12 ›› Issue (03): 201-204.

• 研究论文 • 上一篇    下一篇

高赖氨酸玉米单粒鉴别技术初报——(Ⅱ)—考马斯亮兰印迹法

王育东   

  1. 中国农业科学院作物所
  • 收稿日期:1983-12-16 修回日期:1900-01-01 出版日期:1986-05-12 网络出版日期:1986-05-12

THE SINGLE KERNEL EVALUATION TECHNIQUES FOR THE HIGH-LYSINE CORN(Ⅱ)……COOMASSIE BRILLIANT BIUE STAINING METHOD

Wang Yudong   

  1. Institute of Crop Breeding and Cultivation; Chinese Academy of Agricultural Sciences
  • Received:1983-12-16 Revised:1900-01-01 Published:1986-05-12 Published online:1986-05-12

摘要: 高赖氨酸玉米的单粒鉴别技术不仅要求准确而且要求简便以适应作大量的单粒分析。根据考马斯亮兰 G 250可以定量地与蛋白质相结合的原理,将点有各籽粒的醇溶蛋白提取液的滤纸先用考马斯亮兰染色,经脱色后在滤纸上就可以显现出深浅程度不同的色斑。色斑深的醇溶蛋白含量高赖氨酸含量低;色斑浅的醇溶蛋白含量低而赖氨酸含量高。这样,就可以用肉眼直接挑选出那些高赖氨酸籽粒。

Abstract:

The single kernel evaluation techniques for the high-lysine cornshould be both accurate and simple in order to determine the kernels inlarge quantity.Based on the fact that the Coomassie Brilliant Blue canbe bound to the protein quantitatively,the zein extraction solutionsfrom every kernel are applied to a filter paper,then stained with Coom-assie Brilliant Blue G 250 solution.After destaining the spots of diffe-rent color concentration appears on the filter paper.The dark spots arethe lower lysine kernels,...

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