魔芋茎尖玻璃化冻存研究

作物学报 ›› 2001, Vol. 27 ›› Issue (01): 97-102.

魔芋茎尖玻璃化冻存研究

张玉进;张兴国;庞杰;刘佩瑛

西南农业大学园艺系，重庆400716

收稿日期:1999-09-15 修回日期:2000-05-03 出版日期:2001-01-12 网络出版日期:2001-01-12
通讯作者: 张玉进

Cryopreservation of in Vitro-grown Shoot Tips of Amorphophallus by Vitrification

Zhang Yujin;Zhang Xingguo;Pang Jei;Liu Peiying

Department of Horticulture, Southwest Agricultural University, Chongqing 400716, China

Received:1999-09-15 Revised:2000-05-03 Published:2001-01-12 Published online:2001-01-12
Contact: Zhang Yujin

摘要/Abstract

摘要： 研究了魔芋茎尖玻璃化冻存的主要技术环节，建立了较适宜的魔芋种质资源超低温保存体系：在实体显微镜下，从继代培养2周的不定芽上切取1～1.33mm大小的茎尖，经0.7mol/L蔗糖的MS-NH+4培养液室内预培养2d，玻璃化溶液PVS2室温下处理10～20min，直接投入液氮中保存。保存后，茎尖经40～77℃水浴快速化冻，1.2mol/L蔗糖的培养液

关键词: 玻璃化冻存, 超低温保存, 魔芋

Abstract: The factors affecting shoot tips cryopreservation of Amorphophallus, such as preculture, treatment with PVS2, and thawing temperature were investigated A suitable procedure was established as follows: two weeks after subculture, shoot tips (1～1.33 mm long) were taken from buds which were growing an

Key words: Vitrification, Cryopreservation, Amorphophallus

中图分类号:

S632

张玉进;张兴国;庞杰;刘佩瑛. 魔芋茎尖玻璃化冻存研究[J]. 作物学报, 2001, 27(01): 97-102.

Zhang Yujin;Zhang Xingguo;Pang Jei;Liu Peiying. Cryopreservation of in Vitro-grown Shoot Tips of Amorphophallus by Vitrification[J]. Acta Agron Sin, 2001, 27(01): 97-102.

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