异源三倍体水稻原生质体培养植株再生

作物学报 ›› 1994, Vol. 20 ›› Issue (05): 578-581.

异源三倍体水稻原生质体培养植株再生

张智奇;钟维瑾;唐克轩;周音;祝明福

上海市农业科学院作物育种栽培研究所，上海，201106

收稿日期:1992-05-20 修回日期:1993-12-15 出版日期:1994-09-12 网络出版日期:1994-09-12
通讯作者: 张智奇

Plant Regeneration from Allotriploid (O.sativa×O.minuta) Hybrid Protoplast

Zhang Zhi-qi; Zhong Wei-jin; Tang Ke-xuan; Zhou Yin; Zhu Ming-fu

Crop Breeding and Cultivation Research Institute, SAAS, Shanghai,201106

Received:1992-05-20 Revised:1993-12-15 Published:1994-09-12 Published online:1994-09-12
Contact: Zhang Zhi-qi

摘要/Abstract

摘要： 本试验利用二倍体栽培稻（Oryza stativa L.）品种8204，染色体组型为AA，2n=24作为母本，四倍体小粒野生稻（Oryza minuta）染色体组型为BBCC，2n=4x=48作父本杂交，获得异源三倍体杂交种，染色体组型为ABC。F1根尖检查，染色体数目为2n=3x=36。用该异源三倍体杂交种的幼穗诱导愈伤组织，经长期细代培养进行原生质体游离，获

关键词: 异源三倍体，悬浮培养，琼脂糖包埋，植株再生

Abstract: The experimental material was an allotriploid (ABC 2n=3x=36), the hybrid F1 of the cross O. sativa (AA 2n=24) × O. minuta (BBCC 2n=4x=48). Calli derived from young panicals of hybrid F1 through long-term subculture were used for protoplast isolation and plantlets were regenerated. The results indic

Key words: Allotriploid, Suspention, Agarose embedding, Plant regeneration

张智奇;钟维瑾;唐克轩;周音;祝明福. 异源三倍体水稻原生质体培养植株再生[J]. 作物学报, 1994, 20(05): 578-581.

Zhang Zhi-qi; Zhong Wei-jin; Tang Ke-xuan; Zhou Yin; Zhu Ming-fu. Plant Regeneration from Allotriploid (O.sativa×O.minuta) Hybrid Protoplast[J]. Acta Agron Sin, 1994, 20(05): 578-581.

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