谷子胚性愈伤组织耐盐系的选择及其生理生化特性分析

作物学报 ›› 1994, Vol. 20 ›› Issue (02): 241-247.

谷子胚性愈伤组织耐盐系的选择及其生理生化特性分析

陆卫;贾敬芬

兰州大学细胞研究室，甘肃兰州，730000

收稿日期:1991-10-21 修回日期:1992-08-18 出版日期:1994-03-12 网络出版日期:1994-03-12
通讯作者: 贾敬芬

Selection of NaCl-tolerant Cell Line from embryogenic calli of Millet and Studies on Its Physiological and Biochemical Characteristics

Lu Wei; Jia Jing-fen

Cell Biology Laboratory, Lanzhou, University, 730000

Received:1991-10-21 Revised:1992-08-18 Published:1994-03-12 Published online:1994-03-12
Contact: Jia Jing-fen

摘要/Abstract

摘要： 谷子幼穗诱导形成的愈伤组织，经甲基磺酸乙脂（EMS）诱变处理和8个月的连续筛选及近6个月的稳定培养后，获得了一个耐盐性稳定的细胞变异系，并且获得了它的再生植株。对在无盐压下生长三代的耐盐细胞系的生理生化分析表明，其对氯化钠和聚乙二醇所引起的离子和水分胁迫具有较强的抗性。在无盐胁迫条件下，耐盐系的相对细胞

关键词: 谷子，EMS诱变，耐盐细胞系选择

Abstract: A stable NaCl-tolerant cell line was obtained by treating the embryogenic calli initiated from young inflorescence segments of millet (Setaria italica, Var.7520) with ethylmethane sulphonate (EMS) after screening for 8 months and stabilizing for about 6 months. The regenerated plants were produced f

Key words: Millet, Mutation, NaCl-tolerant cell line

陆卫;贾敬芬. 谷子胚性愈伤组织耐盐系的选择及其生理生化特性分析[J]. 作物学报, 1994, 20(02): 241-247.

Lu Wei; Jia Jing-fen. Selection of NaCl-tolerant Cell Line from embryogenic calli of Millet and Studies on Its Physiological and Biochemical Characteristics[J]. Acta Agron Sin, 1994, 20(02): 241-247.

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