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作物学报

作物学报 ›› 1994, Vol. 20 ›› Issue (03): 257-263.

• 研究论文 •    下一篇

稻瘟病菌株的DNA指纹及其与小种致病性相互关系的研究

朱衡;陈美玲;朱立煌;蒋琬如;王久林;雷财林;凌忠专   

  1. 中国科学院遗传研究所，北京，100101
  • 收稿日期:1992-09-24 修回日期:1993-03-28 出版日期:1994-05-12 网络出版日期:1994-05-12
  • 通讯作者: 朱衡

DNA Fingerprintind and It’s Relationship with the Pathogenicity in Rice Blast Fungus

Zhu Heng; Chen Mei-ling; Zhu Li-huang;Jiang Wan-ru; Wang Jiu-lin; Lei Cai-lin; Ling Zhong-zhuan   

  1. Institute of Gentics, Academia Sinica, Beijing, 100101
  • Received:1992-09-24 Revised:1993-03-28 Published:1994-05-12 Published online:1994-05-12
  • Contact: Zhu Heng

PDF

977

被引次数

69

摘要： 稻瘟病菌生理小种的鉴别对培育抗稻瘟病水稻品种具有重要意义。传统方法利用一套鉴别品种区分小种既复杂又易受各种因素的影响。我们在日本稳定菌株北1的基因组文库中筛选出了一套含重复顺序的克隆，2 个克隆被证实具有高度的多态性。用其中的POR6对17个稻瘟病菌株Southern分析，获得了可分辨和重复的基因组特异的指纹图谱。

关键词: 稻瘟病菌，DNA指纹分析，探针，分子杂交

Abstract: Identification of rice blast fungus is important to breeding blast-resistant rice. The traditional method for assign ment of the blast pathotypes is complicated and its results are vulnerable to the effects of various factors. In order to develop a precise identification method based on DNA fingerpr

Key words: Pyricularia oryzae, DNA fingerprint, Probe, Molecular hybridization

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