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作物学报 ›› 2008, Vol. 34 ›› Issue (04): 571-577.doi: 10.3724/SP.J.1006.2008.00571

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦Glu-1位点变异和1B/1R易位对谷蛋白亚基表达量和面包加工品质的影响

唐建卫1,2;刘建军3;张平平4;肖永贵1;张勇1;曲延英2;何中虎1,5,*   

  1. 1 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/国家小麦改良中心, 北京 100081; 2 新疆农业大学农学院, 新疆乌鲁木齐 830052; 3 山东省农业科学院作物研究所, 山东济南 250100; 4 江苏省农业科学院农业生物技术研究所, 江苏南京 210014; 5 国际玉米小麦改良中心中国办事处, 北京 100081
  • 收稿日期:2007-09-04 修回日期:1900-01-01 出版日期:2008-04-12 网络出版日期:2008-04-12
  • 通讯作者: 何中虎

Effect of Allelic Variation at the Glu-1 Loci and 1B/1R Translocation on the Quantity of Gluten Protein Fractions and Pan Bread Making Quality in Common Wheat

TANG Jian-Wei12,LIU Jian-Jun3,ZHANG Ping-Ping4,XIAO Yong-Gui1,ZHANG Yong1,QU Yan-Ying2,HE Zhong-Hu15*   

  1. 1 Institute of Crop Sciences / National Key Facility for Crop Gene Resource and Genetic Improvement/ National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing 100081; 2 College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, Xinjiang; 3 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong; 4 Institute of Agricultural Biology and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu; 5 CIMMYT China Office, Beijing 100081, China
  • Received:2007-09-04 Revised:1900-01-01 Published:2008-04-12 Published online:2008-04-12
  • Contact: HE Zhong-Hu

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1350

被引次数

22

摘要： 选用北方冬麦区近年来育成的优质强筋品种及山东省主栽品种共42份, 采用反相高效液相色谱法(RP-HPLC)和凝胶色谱法(SE-HPLC)对小麦贮藏蛋白组分进行量化, 分析了不同高分子量谷蛋白亚基(HMW-GS)组成对其表达量、面团流变学特性和面包加工品质的影响。结果表明, Glu-D1位点对谷蛋白亚基含量和加工品质的加性效应最大, 达5%显著水平, 贡献率为28.5%~71.3%。在Glu-A1和Glu-D1位点, 单个亚基对谷蛋白亚基含量和加工品质的贡献分别为1>2*>N和5+10>2+12>4+12, 而在Glu-B1位点, 则表现为差异不显著。不同亚基组合的HMW–GS表达量差异达5%显著水平, 相同亚基组合的品种间贮藏蛋白组分表达量的变异较大, 亚基表达量的差异可能是导致品种间品质差异的重要原因。1B/1R易位显著降低LMW-GS、谷蛋白总量和%UPP, 导致加工品质变劣。选择具有优质亚基组合, 且谷蛋白亚基表达量高的类型, 是有效改良面筋强度, 进一步提高优质新品种选育的有效途径。

关键词: 普通小麦, 贮藏蛋白, 表达量, 面团流变学特性, 1B/1R, HPLC

Abstract: Composition and quantity of gluten protein fractions play an important role in determining the processing quality in common wheat. Wheat varieties with over-expression of HMW glutenin subunit produced very strong and extensible dough. Thus it is very important to understand the relationship between the quantity of gluten protein fractions and dough properties, and pan bread making quality. In this study, 42 wheat genotypes including newly released high quality cultivars and advanced lines in Northern China Winter Wheat Region and leading cultivars in Shandong province were grown in Jinan in 2005–2006 cropping season. Reversed-phase high-performance liquid chromatography (RP-HPLC) and size-exclusion high-performance liquid chromatography (SE-HPLC) were used to analyze the relationship between the quantity of gluten protein fractions and different storage protein compositions, dough properties, and pan bread making quality. The results showed that Glu-D1 locus had the largest effect on the quantity of gluten protein fractions and pan bread making quality in all loci studied, accounting for 28.5%–71.3% of the variation (P<0.05). According to individual glutenin subunit contribution to the expression quantity of gluten protein fractions and pan bread making quality, different glutenin subunit loci could be ranked as: 1>2*>N at Glu-A1; no significant differences at Glu-B1; and 5+10>2+12>4+12 at Glu-D1. Quantity of HMW-GS was significantly different at various loci (P<0.05), and expression quantity of gluten protein fractions showed broad variation among genotypes with the same HMW-GS composition. It indicated that the difference of gluten expression quantity could be an important factor resulting in quality difference in wheat cultivars. 1B/1R translocation caused a significant decrease in expression quantity of LMW-GS, gluten content and %UPP, and showed poor pan bread making quality. Both desirable protein composition and high expression quantity are needed to be considered for efficiently improving dough quality.

Key words: Common wheat, Gluten protein, Expression quantity, Dough property, 1B/1R, HPLC

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