欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2011, Vol. 37 ›› Issue (08): 1360-1371.doi: 10.3724/SP.J.1006.2011.01360

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘肃省50个主要小麦品种(系)苗期抗条锈基因推导及成株期抗病性分析

曹世勤1,2，张勃2，李明菊3,4，徐世昌3，骆惠生2，金社林2,*，贾秋珍2，黄瑾2，金明安2，尚勋武1,*   

  1. 1甘肃农业大学农学院，甘肃兰州 730070；2甘肃省农业科学院植物保护研究所，甘肃兰州 730070；3中国农业科学院植物保护研究所，北京 100193；4云南农业科学院农业环境资源研究所，云南昆明 650205
  • 收稿日期:2011-01-19 修回日期:2011-04-27 出版日期:2011-08-12 网络出版日期:2011-06-13
  • 通讯作者: 金社林, E-mail: jinshelin@163.com; 尚勋武, E-mail: shangxuwu@163.com
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(200903035), 国家科技支撑计划项目(2006BAD08A05), 甘肃省农牧厅农业生物技术与开发项目(GNSW-2007-16)资助。

Postulation of Stripe Rust Resistance Genes and Analysis of Adult Resistance in 50 Wheat Varieties (Lines) in Gansu Province

CAO Shi-Qin1,2,ZHANG Bo2,LI Ming-Ju3,4,XU Shi-Chang3,LUO Hui-Sheng2,JIN She-Lin2,*,JIA Qiu-Zhen2,HUANG Jin2,SHANG Xun-Wu1   

  1. 1College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 2Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 3 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 4 Institute of Agriculture Environment and Resource, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
  • Received:2011-01-19 Revised:2011-04-27 Published:2011-08-12 Published online:2011-06-13
  • Contact: 金社林, E-mail: jinshelin@163.com; 尚勋武, E-mail: shangxuwu@163.com

PDF

1296

被引次数

29

摘要： 选用26个来自国内外具有不同毒性谱的条锈菌菌系，对50个甘肃省主要生产品种(系)及抗源材料进行苗期条锈病抗性鉴定，结合系谱分析，分析推导其所含抗条锈基因，同时对43个品种(系)进行了分子检测。推导分析结果表明，中梁25含有Yr3及未知抗病基因；兰天20含有Yr3a+Yr4a+Yr16及未知抗病基因；Y9220-12含有Yr9+YrCle及未知抗病基因；兰天14、陇原932、陇育216及陇原992含有Yr9及未知抗病基因；陇鉴9343、93保4-4、天选43、贵农22含有Yr10+YrMor；兰天19含有Yr12及未知抗病基因；兰天17、95-111-3、98-178-3-2-4、92R137含有Yr26。分子检测结果发现兰天21等14个品种(系)含有Yr9，兰天17、92R178含有Yr26。其余品种(系)含有未知抗病基因。田间抗性鉴定及监测结果显示，供试品种苗期抗条锈性和成株期抗条锈性结果不完全一致，兰天16等10个品种(系)可能具有成株抗性，兰天14等10个品种(系)可能具有慢条锈性。

关键词: 小麦条锈病, 抗病基因, 基因推导, 分子检测, 甘肃省

Abstract: By means of artificial inoculation of 26 Puccinia striiformis isolates with different spectra of pathogenicity at seedling stage, the resistance to strip rust was identified in 50 wheat varieties (lines) from Gansu province, China. In combination with pedigree analysis and resistance reactions of 30 standard varieties with known resistant genes, the resistance genes in the 50 varieties (lines) were postulated. The resistant genes Yr5, Yr10, Yr15, Yr26, and Yr9 (1B/1R) were validated using molecular markers in 43 varieties (lines). The results of resistance gene postulation showed that Zhongliang 25 was carrying Yr3 and unknown genes. The vareity Lantian 20 was postulated to possess Yr3a, Yr4a, Yr16, and unknown genes. The line Y9220-12 was postulated to possess Yr9, YrCle, and unknown genes. The varieties (lines) Lantian 14, Longyuan 932, Longyu 216, and Longyuan 992 were postulated to possess Yr9 and unknown genes. Genes Yr10 and YrMor were postulated in Longjian 9343, 93-Bao 4-4, Tianxuan 43, and Guinong 22. The variety Lantian 19 was postulated to possess Yr12 and unknown resistant genes. Gene Yr26 was postulated in Lantian 17, 95-111-3, 98-178-3-2-4, and 92R137. Fourteen varieties (lines) were detected to be Yr9 (1B/1R) wheat–rye translocation, which accounted for 32.6% of the total varieties. Yr26 was detected in 92R178 and Lantian 17. Other varieties (lines) were controlled by unknown genes. Although most varieties (lines) tested were highly susceptible at seedling stage, some of them showed adult resistance in the observation pool in Longnan, Gansu, China. Ten varieties (lines) including Lantian 10 might possess adult resistance and another 10 varieties (lines) including Lantian 14 might be varieties with slow rusting characters.

Key words: Wheat stripe rust, Resistance gene, Gene postulation, Molecular detection, Gansu Province of China

[1]Li Z-Q(李振岐), Zeng S-M(曾士迈). Wheat Rust in China (中国小麦锈病). Beijing: China Agriculture Press, 2002 (in Chinese)
[2]Chen W-Q(陈万权), Wang J-X(王剑雄). Genes for leaf and stem rust resistance in 76 wheat genetic resources. Acta Agron Sin (作物学报), 1997, 23(6): 655-663 (in Chinese with English abstract)
[3]Niu Y-C(牛永春), Qiao Q(乔奇), Wu L-R(吴立人). Postulation of resistance genes to stripe rust in commercial wheat cultivars from Henan, Shandong and Anhui provinces. Acta Phytopathol Sin (植物病理学报), 2000, 30(2): 122-128 (in Chinese with English abstract)
[4]Wang F-L(王凤乐), Wu L-R(吴立人), Wan A-M(万安民), Song W-Z(宋位中). Analysis of resistance genes to stripe rust in commercial wheat cultivars from Shanxi, Gansu and Sichuan provinces. Acta Agron Sin (作物学报), 1994, 20(5): 589-594 (in Chinese with English abstract)
[5]Wang F-L(王凤乐), Wu L-R(吴立人), Xie S-X(谢水仙), Wan A-M(万安民). Postulation of genes and adult resistance to stripe rust of Chinese important wheat resistance sources. Acta Phytopathol Sin (植物病理学报), 1994, 24(2): 175-180 (in Chinese with English abstract)
[6]Chen W-Q(陈万权), Qin Q-M(秦庆明), Chen Y-L(陈扬林), Wu Z-X(吴支行), Ma Z-Q(马志强), Liao Q(廖琴). Leaf rust resistance of 40 wheat cultivars in China. Acta Phytopathol Sin (植物病理学报), 2001, 31(1): 16-25 (in Chinese with English abstract)
[7]Chen W-Q(陈万权), Hu C-C(胡长程). Postulation of genes for leaf rust resistance in 28 Chinese wheat. Acta Agron Sin (作物学报), 1993, 19(3): 268-275 (in Chinese with English abstract)
[8]Chen W-Q(陈万权), Qin Q-M(秦庆明), Wang K-R(王奎荣), Xia S-B(夏善保), Feng G-H(冯国华), Chen R-Z(陈荣振). Genes for leaf and stem rust resistance in important wheat cultivars in Jiangsu province. Acta Phytophylacica Sin (植物保护学报), 1997, 24(3): 225-233 (in Chinese with English abstract)
[9]Chen W-Q(陈万权), Wang J-X(王剑雄). Genes for leaf and stem rust resistance in 76 wheat genetic resources. Acta Agron Sin (作物学报), 1997, 23(6): 655-663 (in Chinese with English abstract)
[10]Yuan J-H(袁军海), Liu T-G(刘太国), Chen W-Q(陈万权). Postulation of leaf rust resistance genes in 47 new wheat cultivars (lines) at seedling stage. Sci Agric Sin(中国农业科学), 2007, 40(9): 1925-1935 (in Chinese with English abstract)
[11]Li Z F, Xia X C, Zhou X C, Niu Y C, He Z H, Zhang Y, Li G Q, Wan A M, Wang D S, Chen X M, Lu Q L, Singh R P. Seedling and slow rusting resistances to stripe rust in Chinese common wheat. Plant Dis, 2006, 90: 1302-1312
[12]Francis H A, Leitch A R, Koebner R M D. Conversion of a RAPD-generated PCR product, containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat. Theor Appl Genet, 1995, 90: 636-642
[13]Chen X M, Soria M A, Yan G P, Sun J, Dubcovsky J. Development of sequence tagged site and cleaved amplified polymorphic sequence markers for wheat stripe rust resistance gene Yr5. Crop Sci, 2003, 43: 2058-2064
[14]Shao Y-T(邵映田), Niu Y-C(牛永春), Zhu L-H(朱立煌), Zhai W-X(翟文学), Xu S-C(徐世昌), Wu L-R(吴立人). Identification of all AFLP marker linked to the stripe rust resistance gene Yr10 in wheat. Chin Sci Bull (科学通报), 2001, 46(8): 669-672 (in Chinese with English abstract)
[15]Peng J H, Fahima T, Roeder M S, Huang Q Y, Dahan A, Li Y C, Grama A, Nevo E. High-density molecular map of chromosome region harboring stripe-rust resistance genes YrH52 and Yrl5 derived from wild emmer wheat, Triticum dicoccoides. Genetica, 2000, 109: 199-210
[16]Ma J X, Zhou R H, Dong Y S, Wang L F, Wang X M, Jia J Z．Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers. Euphytica, 2001, 120: 219-226
[17]Wang C M, Zhang Y P, Han D J, Kang Z S, Li G P, Cao A Z, Chen P D. SSR and STS markers for wheat stripe rust resistance gene Yr26. Euphytica, 2008, 159: 359-366
[18]Li F-Q(李峰奇), Han D-J(韩德俊), Wei G-R(魏国荣), Zeng Q-D(曾庆东), Huang L-L(黄丽丽), Kang Z-S(康振生). Molecular detection of stripe rust resistant genes in 126 winter wheat varieties from the Huanghuai wheat region. Sci Agric Sin (中国农业科学), 2008, 41(10): 3060-3069 (in Chinese with English abstract)
[19]Yang W-X(杨文雄), Yang F-P(杨芳萍), Liang D(梁丹), He Z-H(何中虎), Shang X-W(尚勋武), Xia X-C(夏先春). Molecular characterization of slow-rusting genes Lr34/Yr18 in Chinese wheat cultivars. Acta Agron Sin (作物学报), 2008, 34(7): 1109-1103 (in Chinese with English abstract)
[20]Bai Y-L(白玉路), Sun Q(孙权), Zhang C-Y(张春宇), Cui N(崔娜), Lin F(林凤), Xu S-C(徐世昌), Zhang Z-Y(章振羽), Gao Y(高阳), Xu X-D(徐晓丹). Molecular detection and resistance evaluation of 59 cultivars from the Northwest of the United States to Chinese stripe rust races. Sci Agric Sin(中国农业科学), 2010, 43(6): 1147-1155 (in Chinese with English abstract)
[21]Wu L(伍玲), Tan J(谭君), Zhu H-Z(朱华忠), Wang Z-L(王中烈), Pu X-R(蒲晓蓉). Detection of stripe rust resistant genes of Yr5, Yrl0 and Yr15 in some Sichuan wheat lines by molecular markers. Southwest China J Agric Sci (西南农业学报), 2007, 20(2): 316-320 (in Chinese with English abstract)
[22]Li Z-Q(李振岐), Shang H-S(商鸿生). Wheat Rusts and Disease Control (小麦锈病及其防治). Shanghai: Shanghai Sciences and Technology Publisher, 1989 (in Chinese)
[23]Dubin H J, Johnson R, Stubbs R W. Postulated genes for resistance to stripe rust in selected CIMMYT and related wheat. Plant Dis, 1989, 73: 472-475
[24]Browde L E. Specificity of the Puccinia recondita f. sp. tritici: Triticum aestivum ‘Bulgaria 88’ relationship. Phytopathology, 1973, 63: 524-528
[25]Han B(韩冰), Lin R-M(蔺瑞明), Cao Y-Y(曹远银), Xu S-C(徐世昌). Comparison of the methods of isolating wheat yellow rust genomic DNA. Chin Agric Sci Bull (中国农学通报), 2006, 22(4): 81-83 (in Chinese with English abstract)
[26]Wu L-R(吴立人), Niu Y-C(牛永春). Strategies of sustainable control of wheat stripe rust in China. Sci Agric Sin (中国农业科学), 2000, 33(5): 1-7 (in Chinese with English abstract)
[27]Wan A M, Zhao Z H, Chen X M, He Z H, Jin S L, Jia Q Z, Yao G, Yang J X, Wang B T, Li G B, Bi Y Q, Yuan Z Y. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Dis, 2004, 88: 896-904
[28]Cao S-Q(曹世勤), Jin S-L(金社林), Jin M-A(金明安), Jia Q-Z(贾秋珍), Li J-P(李继平). Identification of wheat varieties (lines) for resistance to stripe rust in 1994-2002. J Plant Genet Resour(植物遗传资源学报), 2003, 4(2): 119-122 (in Chinese with English abstract)
[29]Li G Q, Li Z F, Yang W Y, Zhang Y, He Z H, Xu S C, Singh R P, Qu Y Y, Xia X C. Molecular tagging of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26. Theor Appl Genet, 2006, 112: 1434-1440
[30]Liu T G., Peng Y L, Chen W Q, Zhang Z Y. First detection of virulence in Puccinia striiformis f. sp. tritici in China to resistance genes Yr24 (=Yr26) present in wheat cultivar Chuanmai 42. Plant Dis, 2010, 94: 1163 (Abstract)
[31]Cao S-Q(曹世勤), Luo H-S(骆惠生), Wu C-P(武翠平), Jin S-L(金社林), Wang X-M(王晓鸣), Zhu Z-D(朱振东), Jia Q-Z(贾秋珍), Huang J(黄瑾), Zhang B(张勃), Shang X-W(尚勋武). Postulation of powder mildew resistance genes in 64 wheat cultivars (lines) in Gansu Province. Acta Agron Sin (作物学报), 2010, 36(12): 2107-2115 (in Chinese with English abstract)
[32]Wang K-N(汪可宁), Wu L-R(吴立人), Meng Q-Y(孟庆玉), Xie S-X(谢水仙), Lu D-Y(路端谊), Yuan W-H(袁文焕), Yu X-R(于孝如), Song W-Z(宋位中), Liu S-J(刘守俭), Wang J-Q(王吉庆), Yang J-X(杨家秀), Huang G-M(黄光明), Li Y-F(李艳芳), Yang S-C(杨世诚). On the physiologic specialization of stripe rust of wheat in China during the years 1975-1984. Acta Phytopathol Sin (植物病理学报), 1986, 16(2): 74-85 (in Chinese with English abstract)
[33]Wang J-Q(王吉庆), Lu J-X(陆家兴), Liu S-J(刘守俭), Dai S-K(戴书琨), Liu R-Z(刘汝祯). A preliminary study on the trend of over summer of stripe rust of wheat in Gansu province. Acta Phytopathol Sin (植物病理学报), 1965, 8(1): 1-10 (in Chinese with English abstract)
[34]Li Z-Q(李振岐),Wang M-N(王美楠), Jia M-G(贾明贵), Lu H-P(路和平), Shang H-S(商鸿生), Kang Z-S(康振生). Study on the epidemiology and control strategy of wheat stripe rust in south Gansu. Acta Univ Agric Boreali-Occident (西北农业大学学报), 1997, 25(2): 1-5 (in Chinese with English abstract)
[35]Li Z-Q(李振岐), Shang H-S(商鸿生), Yin S-L(阴省林), Qiang Z-F(强中发), Zhao Y-Q(赵宜谦), Lu H-P(陆和平), Hong X-W(洪锡午), Song W-Z(宋位中), Liu S-J(刘守俭). Studies on the breakdown of resistance of Lovrin cultivars of wheat to stripe rust (Puccinia striiformis West). Sci Agric Sin (中国农业科学), 1984, 8(1): 68-73 (in Chinese with English abstract)
[36]Xie S-X(谢水仙), Wan A-M(万安民), Wu L-R(吴立人), Chen Y-L(陈扬林). Studies of stripe rust spread on autumn wheat in south Gansu Province. Plant Prot(植物保护), 1994, 20(5): 6-8 (in Chinese with English abstract)
[37]Xie S-X(谢水仙), Chen W-Q(陈万权), Chen Y-L(陈扬林), Song W-Z(宋位中), Liu S-J(刘守俭), Yang F-R(杨发荣), Yang S-F(杨世发), Hu J-W(胡箭卫), Dong J-S(董敬生). Occurrence and management of wheat stripe rust (Puccinia striiformis West) in autumn, winter and spring in the south of Gansu Province. Acta Phytophylacica Sin (植物保护学报), 1997, 21(3): 29-34 (in Chinese with English abstract)
[38]Xie S-X(谢水仙), Wan A-M(万安民), Zhang Q-Q(张庆勤), Jin S-L(金社林), Li J-P(李继平), Cao S-Q(曹世勤). Identification and screening of new germplasm resources of wheat resistance to stripe rust and powdery mildew diseases. Plant Prot (植物保护), 1997, 23(6): 3-5 (in Chinese with English abstract)
[1] 朱峥, 王田幸子, 陈悦, 刘玉晴, 燕高伟, 徐珊, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻转录因子WRKY68在Xa21介导的抗白叶枯病反应中发挥正调控作用[J]. 作物学报, 2022, 48(5): 1129-1140.
[2] 习玲, 王昱琦, 朱微, 王益, 陈国跃, 蒲宗君, 周永红, 康厚扬. 78份四川小麦育成品种(系)条锈病抗性鉴定与抗条锈病基因分子检测[J]. 作物学报, 2021, 47(7): 1309-1323.
[3] 张欢, 罗怀勇, 李威涛, 郭建斌, 陈伟刚, 周小静, 黄莉, 刘念, 晏立英, 雷永, 廖伯寿, 姜慧芳. 花生全基因组抗病基因鉴定及其对青枯菌侵染的响应分析[J]. 作物学报, 2021, 47(12): 2314-2323.
[4] 赵旭阳, 姚方杰, 龙黎, 王昱琦, 康厚扬, 蒋云峰, 李伟, 邓梅, 李豪, 陈国跃. 青藏春冬麦区93份小麦地方种质条锈病抗性评价及抗病基因分子鉴定[J]. 作物学报, 2021, 47(10): 2053-2063.
[5] 白宗璠,竞霞,张腾,董莹莹. MDBPSO算法优化的全波段光谱数据协同冠层SIF监测小麦条锈病[J]. 作物学报, 2020, 46(8): 1248-1257.
[6] 张雪翠,钟超,段灿星,孙素丽,朱振东. 大豆品种郑97196抗疫霉病基因RpsZheng精细定位[J]. 作物学报, 2020, 46(7): 997-1005.
[7] 向文扬,杨永庆,任秋燕,晋彤彤,王丽群,王大刚,智海剑. 大豆抗SC3候选基因的克隆及分析[J]. 作物学报, 2019, 45(12): 1822-1831.
[8] 肖湘谊,史学涛,盛浩闻,刘金灵,肖应辉. 水稻抗稻瘟病基因Pi47的精细定位和候选基因分析[J]. 作物学报, 2018, 44(7): 977-987.
[9] 李晓那,孙石,钟超,韩天富. 黄淮海地区大豆主栽品种对8个大豆疫霉菌株的抗性评价[J]. 作物学报, 2017, 43(12): 1774-1783.
[10] 朱亚军,孙强,王金明,陈凯,冯博,方雅洁,林秀云,徐建龙*. 粳稻品种吉粳809的稻瘟病抗性基因分析[J]. 作物学报, 2016, 42(11): 1638-1646.
[11] 李文凤,王晓燕,黄应昆*,张荣跃,单红丽,罗志明,尹炯. 101份中国甘蔗主要育种亲本褐锈病抗性鉴定及Bru1基因的分子检测[J]. 作物学报, 2016, 42(09): 1411-1416.
[12] 管昌英,郭军,薛凤博,张广旭,王宏伟,李安飞,孔令让. 普通小麦DH155抗白粉病基因的分子作图及应用分子标记辅助选择将其转移[J]. 作物学报, 2015, 41(08): 1183-1190.
[13] 李文凤,王晓燕,黄应昆*,张荣跃,单红丽,尹炯,罗志明. 31份甘蔗野生核心种质资源褐锈病抗性鉴定及Bru1基因的分子检测[J]. 作物学报, 2015, 41(05): 806-812.
[14] 王仲怡,付海宁,孙素丽,段灿星,武小菲,杨晓明,朱振东. 豌豆品系X9002抗白粉病基因鉴定[J]. 作物学报, 2015, 41(04): 515-523.
[15] 宋伟,孙会改,孙艳玲,赵紫慧,王晓鸣,武小菲,李洪杰. 小麦品种汶农14抗白粉病基因的染色体定位[J]. 作物学报, 2014, 40(05): 798-804.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2