欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2013, Vol. 39 ›› Issue (03): 381-388.doi: 10.3724/SP.J.1006.2013.00381

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

甜菜单体附加系M14种子形成方式

马兰1,2,**,杜洪岩1,**,李荣田1,*   

  1. 1 黑龙江大学分子生物学省高校重点实验室, 黑龙江哈尔滨 150080; 2 黑龙江省农业科学院大庆分院, 黑龙江大庆 163000
  • 收稿日期:2012-06-25 修回日期:2012-11-16 出版日期:2013-03-12 网络出版日期:2013-01-04
  • 通讯作者: 李荣田, E-mail: lirongtian07@yahoo.com.cn, Tel: 0451-86608243
  • 基金资助:

    本研究由国家转基因研究与产业化开发专项(JY-03A-24-02)和黑龙江省研究生创新科研项目(YJSCX2009-017HLJ)资助

Seed-Setting Mode of Monosomic Addition Line M14 of Sugar Beet Beta corolliflora

MA Lan1,2,**,DU Hong-Yan1,**,LI Rong-Tian1,*   

  1. 1 Key Laboratory of Molecular Biology, Heilongjiang University, Harbin150080, China; 2 Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163000, China
  • Received:2012-06-25 Revised:2012-11-16 Published:2013-03-12 Published online:2013-01-04
  • Contact: 李荣田, E-mail: lirongtian07@yahoo.com.cn, Tel: 0451-86608243

RichHTML

PDF

1071

被引次数

3

摘要：

以具有显性标记性状的红叶柄栽培甜菜为父本与绿叶柄M14甜菜杂交，观察F1植株叶柄颜色；利用SSR标记分析甜菜M14世代间的遗传相关性。结果显示，甜菜单体附加系M14传递率维持在较高水平；在人工去雄授粉条件下，F1代植株叶柄颜色均为红色，甜菜M14通过接受外来遗传物质及精卵结合的有性生殖方式繁殖种子；M14株系中不存在与其母本株SSR标记基因型精确相同的植株。表明甜菜M14通过精卵融合的有性生殖而非无融合生殖形成种子。Southern blot结果显示，从甜菜M14反转得到的栽培甜菜染色体中普遍含有白花甜菜DNA，暗示甜菜M14形成雌配子体及卵子过程中存在减数分裂过程和较高频率的染色体畸变。推测甜菜单体附加系M14高频传递现象可能是由于附加的白花甜菜第9号染色体具有杀配子功能。

关键词: 甜菜单体附加系M14, 无融合生殖, 有性生殖, 有性杂交, 遗传相关, Southern杂交

Abstract:

A cross combination of M14 with green petiole ×B. vulgaris with red petiole was prepared to observe the dominant trait marker of hybridplants，and thegenetic correlation between the maternal plant and F1 generation of M14 based on the SSR analysis. The result showed that M14 had higher transmission rate of the alien chromosome. Multiplied seeds came from the union of male and female gametes under artificial pollination, and the petiole color of all the hybrid plants was red. There was no the exactly identical genotype in the progeny compared with the maternal plant. It indicated that the seed-setting mode of M14 is not apomixis, but sexual reproduction. Southern blot showed that the chromosomes of B. vulgaris reversed from M14 sharing the DNA sequence ofB. corolliflora,it is implying that there was popular meiosis and chromosome aberration during the female gamete formation of sugar-beet M14. The study came to the conclusion that higher transmission frequency of the alien chromosome of sugar-beet M14 is probably induced by the chromosome 9 of Beta corolliflora with gametocidal function.

Key words: Monosomic addition line M14 in sugar beet, Apomixis, Sexual reproduction, Hybridization, Genetic correlation, Southern blot

[1]Bicknell R A, Koltunow A M. Understanding apomixis: recent advances and remaining conundrums. Plant Cell, 2004, 16: 228–245



[2]Vielle-Calzada J P, Charles F G, David M S. Apomixis: the asexual revolution. Science, 1996, 274: 1322–1323



[3]Fang X H, Gu S H, Xu Z Y, Chen F, Guo D D. Construction of a binary BAC library for an apomictic monosomic addition line of Beta corolliflora in sugar beet and identification of the clones derived from the alien chromosome. Theor Appl Gent, 2004, 108: 1420–1425



[4]Yu B(于冰), Li H-Y(李海英), Ma C-Q(马春泉), Zhang S-J(张绍军), Li R-T(李荣田), Guo D-D(郭德栋). Construction of the subtractive cDNA library of buds of the apomictic monosomic addition line in sugar beet. High Technol Lett (高技术通讯), 2006, 16(9): 954–958 (in Chinese with English abstract)



[5]Ma C-Q(马春泉), Zhang Y(张莹), Cui Y(崔颖), Li H-Y(李海英). Construction of cDNA library in florescence and screening specifically expressed genes in sugar beet M14. Bull Bot Res (植物研究), 2008, 28(4): 408–411(in Chinese with English abstract)



[6]Ge Y, He G C, Guo D D, Li R T. GISH and BAC-FISH study of apomictic Beta M14. Sci in China: Series C Life Sci, 2007, 50: 242–250



[7]Ma C, Wang Y, Wang L, Chen S, Li H. Identification of a sugar beet BvM14-MADS box gene through differential gene expression analysis of monosomic addition line M14. J Plant Physiol, 2011, 168: 1980–1986



[8]Li H Y, Cao Y G, Wang Y G, Ma C Q, Chen S X. Proteomic analysis of sugar beet apomictic monosomic addition line M14. J Proteomics, 2009, 73: 297–308



[9]Zhu H, Bi Y D, Yu L J, Guo D D, Wang B C. Comparative proteomic analysis of apomictic monosomic addition line of Beta corolliflora and Beta vulgaris L. in sugar beet. Mol Biol Rep, 2009, 36: 2093–2098



[10]Guo D-D(郭德栋), Li S-X(李盛贤), Jia S-B(贾树彪), Kang C-H(康传红), Li Y(李勇). Studies on the interspecific hybridization in genus Beta. Sugar Crops China (中国糖料), 1999, (2): 1–8 (in Chinese with English abstract)



[11]Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍), Kang C-H(康传红), Li Y(李勇), Wang G-Z(王桂芝). Acquisition and identification of monosomic additional lines of apomictic beet. J Yunnan Univ (云南大学学报), 2000, 34(5): 1–3 (in Chinese with English abstract)



[12]Guo D-D(郭德栋), Liu L-P(刘丽萍), Kang C-H(康传红). The transmission and reproductive characters of apomictic monosomic addition lines of Beta vulgaris L. J Nat Sci Heilongjiang Univ (黑龙江大学自然科学学报), 2001, 18(3): 104–107 (in Chinese with English abstract)



[13]Gao D-J(高东杰), Kang C-H(康传红), Liu L-P(刘丽萍), Guo D-D(郭德栋), Fang X-H(方晓华). A full set of monosomic addition lines in Beta vulgaris from B. corolliflora. Sugar Crops China (中国糖料), 2001, (4): 5–9 (in Chinese with English abstract)



[14]Jassem B. Apomixis in the Genus Beta. Apomixis Newslett, 1990, (2): 7–23



[15]Kang C-H(康传红), Wang G-Z(王桂芝), Jia S-B(贾树彪), Guo D-D(郭德栋), Liu L-P(刘丽萍). Observation on apomixes of progenies of interspecific hybridization between Beta vulgaris L. and Beta corollifloro. China Sugarbeet (中国甜菜), 1995, (1): 3–8 (in Chinese with English abstract)



[16]Li Y(李勇), Liu W(刘威), Kang C-H(康传红), Jia S-B(贾树彪), Guo D-D(郭德栋). Morphological and isozyme variations of apomictic lines from hybrid progenies between B. vulgaris L. and B. corollifloro. Sugar Crops China (中国糖料), 1997, (2): 6–9 (in Chinese with English abstract)



[17]Guo D-D(郭德栋), Kang C-H(康传红), Liu L-P(刘丽萍), Li Y(李勇), Fang X-H(方晓华). Study of apomixis in the allotriploid beet (VVC). Sci Agric Sin (中国农业科学), 1999, 32(4): 1–5 (in Chinese with English abstract)



[18]Kang C-H(康传红), Liu L-P(刘丽萍), Shen Y(申业), Guo D-D(郭德栋), Fang X-H(方晓华). Studies on apomict transmission rate in allotriploid beet (VVC). Sugar Crops China (中国糖料), 1999, (3): 1–5 (in Chinese with English abstract)



[19]Guo D-D(郭德栋), Li S-X(李盛贤), Jia S-B(贾树彪), Kang C-H(康传红), Li Y(李勇). Studies on the Interspecific Hybridization in Genus Beta. Sugar Crops China (中国糖料), 1999, (2): 1–6 (in Chinese with English abstract)



[20]Shen Y(申业), Shen J-H(申家恒), Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍). Dynamics of callose deposition in cell wall during megasporogenesis in the apomictic monosomic addition line M14 of Beta corolliflora of sugar beet. Acta Agron Sin (作物学报), 2006, 32(6): 894–898 (in Chinese with English abstract)



[21]Shen Y(申业), Shen J-H(申家恒), Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍). Cyto-embryological study of the apomictic monosomic addition line M14 in sugar beet. Acta Bot Boreali-Occident Sin (西北植物学报), 2006, 26(5): 957–963 (in Chinese with English abstract)



[22]Shen Y(申业), Shen J-H(申家恒), Guo D-D(郭德栋), Fang X-H(方晓华), Liu L-P(刘丽萍). Megasporogenesis and development of female gametophyte in the apomictic monosomic addition line M14 of Beta corolliflora in sugar beet. J Wuhan Bot Res (武汉植物学研究), 2006, 24(2): 106–113 (in Chinese with English abstract)



[23]Ding C-H(丁常宏), Shen J-H(申家恒), Guo D-D(郭德栋), Shang Y-J(尚娅佳). Ultrastructure of megasporogenesis in facultative apomictic monosonic addition line M14 of Beta corolliflora of sugar beet. Acta Agron Sin (作物学报), 2009, 35(8): 1516−1524 (in Chinese with English abstract)



[24]Shang Y-J(尚娅佳), Shen J-H(申家恒), Guo D-D(郭德栋), Ding C-H(丁常宏). Ultrastructural characterization of the mature embryo sac in the chromosome-9 monosomic addition line of Beta corolliflora in sugar beet. Chin Bull Bot (植物学报), 2009, 44(6): 682–693 (in Chinese with English abstract)



[25]Shang Y-J(尚娅佳), Shen J-H(申家恒), Guo D-D(郭德栋), Ding C-H(丁常宏). The ultrastructural changes of female gametophyte during Its development in the apomictic monosomic addition line M14 of Beta corolliflora in sugar beet. Sci Agric Sin (中国农业科学), 2010, 43(1): 29–38 (in Chinese with English abstract)



[26]Gao D J, Schmidt T, Jung C. Molecular characterization and chromosomal distribution of species-specific repetitive DNA sequences from Beta corolliflora, a wild relative of sugar beet. Genome, 2000, 43: 1073–1080



[27]Li R T, Zhang Z M, Zhang Q F. Transformation of japonica rice with RHL gene and salt tolerance of the transgenic rice plant. Chin Sci Bull (科学通报), 2002, 47(8): 613–617



[28]Maguire M P. High transmission frequency of a tripsacum chromosome in corn. Genetics, 1963, 48: 1185–1194



[29]Nogler G A. Genetics of apomictic in Ranuncuclu auricomus: VI. Epilogue. Bot Helvet, 1995, 105, 111–115



[30]Bracaccia G, Mazzucato A, Belardinelli A. Inheritance of parental genomes in progenies of Poa pratensis L. from sexual and apomictic genotypes as assessed by RAPD markers and flow cytometry. Theor Appl Genet, 1997, 95: 516–524



[31]Kang C-H(康传红), Han X-Y(韩晓云), Wang Z-W(王志伟), Guo D-D(郭德栋), Wang G-Z(王桂芝). RAPD analysis to confirm the identification of three progenies of M14 beta vulgaris additional lines. Biotechnology (生物技术), 2002, (4): 9–11 (in Chinese with English abstract)



[32]Endo T R. Gametocidal chromosomes and their induction of chromosome mutations in wheat. Jpn J Genet, 1990, 65: 135–152
[1] 曹新川,胡守林,韩秀锋,何良荣,郭伟锋. 海岛棉棉铃阶段性发育与产量品质的关系[J]. 作物学报, 2020, 46(02): 300-306.
[2] 黄中文,徐新娟,王伟,梅沛沛. 河南夏大豆区近30年主要大豆品种产量改良的遗传进展[J]. 作物学报, 2016, 42(07): 1009-1015.
[3] 张高阳,祁建民,徐建堂,牛小平,张雨佳,张立武,陶爱芬,方平平,林荔辉. 圆果黄麻纤维素合成酶基因CcCesA1的克隆、反义载体构建及转化拟南芥[J]. 作物学报, 2014, 40(05): 816-822.
[4] 丁常宏，申家恒，郭德栋，尚娅佳，陆俊萍. 甜菜无融合生殖单体附加系M14大孢子发生的超微结构[J]. 作物学报, 2009, 35(8): 1516-1524.
[5] 邬亚文;于永红;胡国成;傅亚萍;斯华敏;郭泽建;孙宗修. 一个新的水稻生育期延迟T-DNA插入突变体[J]. 作物学报, 2006, 32(08): 1111-1116.
[6] 吴杨;周会;潘大仁. 甘蔗线虫病抗性基因的PCR检测研究[J]. 作物学报, 2006, 32(06): 939-942.
[7] 申业; 申家恒; 郭德栋 ;方晓华 ; 刘丽萍. 甜菜无融合生殖单体附加系M14大孢子发生期间细胞壁胼胝质的变化[J]. 作物学报, 2006, 32(06): 894-898.
[8] 平俊爱;张福耀;崔贵梅;程庆军;杜志宏;张义贤. 高粱无融合生殖系2083自主结实特性及胚胎学研究[J]. 作物学报, 2004, 30(07): 714-718.
[9] 张名位;郭宝江;彭仲明. 籼型黑米稻粒形性状与其中某些矿质元素含量的遗传相关性[J]. 作物学报, 2003, 29(02): 305-310.
[10] 陈光辉;官春云; 陈立云; 康春林. 两系亚种间杂交稻籽粒充实度的遗传研究[J]. 作物学报, 2001, 27(06): 811-816.
[11] 母锡金;蔡雪;孙德兰;时光春;朱至清. 被子植物的无融合生殖和它的应用前景[J]. 作物学报, 2001, 27(05): 590-599.
[12] 蔡得田;袁隆平;卢兴桂. 二十一世纪水稻育种新战略[J]. 作物学报, 2001, 27(01): 110-116.
[13] 张福耀;B.L.Burson;J.Actkinson;韩雪梅;崔贵梅. 谷子(Setaria italica Beauv)无融合生殖系SMA-1的发现[J]. 作物学报, 2001, 27(01): 65-70.
[14] 高建伟;孙其信;孙振山. 小麦与无融合生殖披碱草(Elymus rectisetus)属间杂种F1的形态学和细胞遗传学研究[J]. 作物学报, 2000, 26(03): 271-277.
[15] 郑德英;唐龙飞;章宁;陈坚. 满江红有性杂交研究及其鉴定[J]. 作物学报, 1994, 20(06): 701-709.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2